数字农科院2.0

Distribution and transmission of β-lactamase resistance genes in meal-to-milk chain on dairy farm

文献类型： 外文期刊

作者： Zheng Qi;Shuhan Jin;Xiaorui Guo;Hailong Tong;Nanqi Ren;Shijie You

作者机构：

关键词： Antibiotic resistance genes;Meal-to-milk chain;Mobile genetic elements;β-lactamase resistance genes

期刊名称： Environmental Pollution

ISSN： 0269-7491

年卷期： 2023 年 331 卷

页码：

收录情况： SCIE（2023版） ; ; EI（2023版）

摘要： Antibiotics have been widely used in animal husbandry, which leads to high risk of food-borne transfer of antibiotic resistance genes (ARGs). The present study investigated the distribution of β-lactamase resistance genes (β-RGs) on dairy farm in the Songnen Plain of western Heilongjiang Province, China, to provide mechanistic insights into food-borne transmission of β-RGs through “meal-to-milk” chain under practically relevant circumstances. The results demonstrated that the abundance of β-RGs (91%) was much higher than that of other ARGs in the livestock farms. The blaTEM exhibited the content as high as 94.55% among all ARGs, and higher than 98% blaTEM was detected in meal, water and milk sample. The metagenomic taxonomy analysis indicated that the blaTEM should be carried by tnpA-04 (7.04%) and tnpA-03 (1.48%) hosted in Pseudomonas genus (15.36%) and Pantoea (29.02%) genus. Both tnpA-04 and tnpA-03 in the milk sample were identified to be the key mobile genetic elements (MGEs) responsible for transferring blaTEM along the “meal-manure-soil-surface water-milk” chain. The ARGs transfer across ecological boundaries underscored the need to evaluate potential dissemination of high-risk Proteobacteria and Bacteroidetes carried by humans and animals. They were capable of producing expanded-spectrum β-lactamases (ESBLs) and destroying commonly used antibiotics, leading to possible risk of food-borne horizontal transmission of ARGs. This study not only has important environmental implications for identifying the pathway for ARGs transfer, but also highlights the demand for appropriate policy toward safe regulation of dairy farm and husbandry products.

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