数字农科院2.0

The Nlr Protein Encoded By The Resistance Genety-2Is Triggered By The Replication-Associated Protein Rep/C1 Of Tomato Yellow Leaf Curl Virus

文献类型： 外文期刊

作者： Shen, XX; Yan, Z; Wang, XX; Wang, YL; Arens, M; Du, YC; Visser, RGF; Kormelink, R; Bai, YL; Wolters, AMA

作者机构：

关键词： Tylcv; Ty-2; Cloning; Nlr Gene; Rep; C1; Avirulence Factor

期刊名称： FRONTIERS IN PLANT SCIENCE

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： The whitefly-transmitted tomato yellow leaf curl virus (TYLCV) is one of the most destructive viral pathogens of cultivated tomato. To combat TYLCV, resistance geneTy-2has been introduced into cultivated tomato (Solanum lycopersicum) from wild tomato speciesSolanum habrochaitesby interspecific crossing. Introgression lines withTy-2contain a large inversion compared withS. lycopersicum, which causes severe suppression of recombination and has hampered the cloning ofTy-2so far. Here, we report the fine-mapping and cloning ofTy-2using crosses between aTy-2introgression line and several susceptibleS. habrochaitesaccessions.Ty-2was shown to encode a nucleotide-binding leucine-rich repeat (NLR) protein. For breeding purposes, a highly specific DNA marker tightly linked to theTy-2gene was developed permitting marker-assisted selection. The resistance mediated byTy-2was effective against the Israel strain of TYLCV (TYLCV-IL) and tomato yellow leaf curl virus-[China : Shanghai2] (TYLCV-[CN : SH2]), but not against tomato yellow leaf curl Sardinia virus (TYLCSV) and leafhopper-transmitted beet curly top virus (BCTV). By co-infiltration experiments we showed that transient expression of the Rep/C1 protein of TYLCV, but not of TYLCSV triggered a hypersensitive response (HR) inNicotiana benthamianaplants co-expressing theTy-2gene. Our results indicate that theRep/C1gene of TYLCV-IL presents the avirulence determinant ofTy-2-mediated resistance.

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