数字农科院2.0

Harnessing Transient Expression Systems with Plant Viral Vectors for the Production of Biopharmaceuticals in Nicotiana benthamiana

文献类型： 外文期刊

作者： Sayed Abdul Akher;Kevin Yueju Wang;Kylie Hall;Oluwaseyi Setonji Hunpatin;Muhammad Shan;Zenglin Zhang;Yongfeng Guo

作者机构：

关键词： biopharmaceuticals;controlled environment agriculture;N. benthamiana;plant molecular farming;recombinant proteins;transient expression

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2025 年 26 卷 12 期

页码：

收录情况： SCIE（2025版）

摘要： Plant Molecular Farming (PMF) capitalizes on the unique properties of plants as bioreactors to efficiently produce valuable proteins, pharmaceuticals, and enzymes. This review emphasizes the critical role of transient expression systems, particularly in Nicotiana benthamiana, due to its susceptibility to various pathogens. Viral vector-based transient expression has proven essential during health emergencies like COVID-19, enabling rapid recombinant protein production. The review also evaluates different transient expression platforms and highlights their applications in biopharmaceutical production, education, synthetic biology, and gene editing. Advances in viral vector modification, hydroponics, and Controlled Environment Agriculture (CEA) are presented as transformative innovations enhancing scalability and regulatory compliance. Furthermore, glycoengineering advancements broaden the range of producible biopharmaceuticals, improving global medication access. By exploring these advancements, this review underscores the vast potential of transient expression systems to meet dynamic scientific and market demands, positioning PMF as a vital component in modern biotechnology.

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