数字农科院2.0

Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β

文献类型： 外文期刊

作者： Mengmeng Xu;Mengmeng Huang;Guodong WANG;Jingzhe HAN;Xinxin NIU;Jinze HAN;Hangbo YU;Yulong ZHANG;Runhang LIU;Ziwen WU;Hongyu Cui;Yanping Zhang;Suyan Wang;Yongzhen Liu;Yuntong Chen;Yulu Duan;Liuan Li;Yulong Gao;Xiaole Qi

作者机构：

关键词： Antigenic epitope;Chicken;Detection;Monoclonal antibody;Pro-IL-1β

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2025 年 104 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1β, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1β monoclonal antibody (mAb). The secreted mAb 1E12 can recognize exogenous or endogenous chicken pro-IL-1β by Western blot and IFA techniques, and for the first time, a novel antigen epitope 13SSLSEETFY21 of chicken pro-IL-1β recognized by this mAb was identified. This study not only provides an important tool for the detection and research of chicken pro-IL-1β, but also has significant implications for understanding the antigenic structure and biochemical characteristics of chicken pro-IL-1β.

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