数字农科院2.0

Identification and Nontargeted Screening of Phytosterol Esters in Oilseeds and Oils Based on Fragmentation Characteristics via Ultrahigh-Performance Liquid Chromatography High-Resolution Mass Spectrometry

文献类型： 外文期刊

作者： Zhao, Ya;Wang, Xiupin;Li, Peiwu

作者机构：

关键词： fragmentation rules and database;nontargeted screening;oilseeds and oils;phytosterol esters;ultrahigh-performance liquid chromatography-high resolution mass spectrometry

期刊名称： FOOD FRONTIERS

ISSN：

年卷期： 2025 年

页码：

收录情况： EI（2025版） ; ; ESCI（2025版）

摘要： Phytosterol (PS) esters are natural bioactive compounds with various physiological functions, such as reducing cholesterol levels and protecting cardiovascular health. In the present study, a novel method for identifying and screening phytosterol esters (PSEs) was developed by ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry. First, the structural characteristics, mass spectrometry behaviors, and fragmentation rules of PSEs were investigated and summarized. An in-house theoretical database was then established. The database contained 1,176 PSEs, including their chemical formulas, exact molecular masses, protonated forms, accurate masses of precursor ions, and characteristic fragment ions. A nontargeted screening workflow was then developed to identify unknown PSEs and distinguish isomers with different fatty acid acyl chain lengths based on precursor ions and characteristic fragments. To validate the applicability of the method, the most effective extraction method (Matyash) for PSEs was determined, and this nontargeted screening was applied to 23 types of oilseeds and oils from China. Ultimately, 12 PSEs were identified, and their compositional characteristics in these samples were analyzed. This innovative approach not only advances the analytical techniques for PSEs in oilseeds and oils but also lays a foundation for future research on the nutritional profiling of various food substrates, potentially facilitating the development of functional foods aimed at enhancing human health.

分类号：

  • 相关文献

[1]Production of Novel "Functional Oil" Rich in Diglycerides and Phytosterol Esters with "One-Pot" Enzymatic Transesterification. Zheng, Ming-Ming,Huang, Qing,Huang, Feng-Hong,Guo, Ping-Mei,Xiang, Xia,Deng, Qitan-Chun,Li, Wen-Lin,Wan, Chu-Yun,Zheng, Chang.

[2]Ultrasonic pretreatment for lipase-catalyed synthesis of phytosterol esters with different acyl donors. Zheng, Ming-Ming,Wang, Lian,Huang, Feng-Hong,Dong, Ling,Guo, Ping-Mei,Deng, Qian-Chun,Li, Wen-Lin,Zheng, Chang.

[3]Deacidification of high-acid rice bran oil by the tandem continuous-flow enzymatic reactors. Liujia Xu,Yi Zhang,Vladimir Zivkovic,Mingming Zheng. 2022

[4]The Mesoporous Polydopamine-Based Bowl-like Lipase Nanoreactor for the Biocatalytic Synthesis of Oleate Sterol Ester. Hou, Huaqing,Zhang, Shan,Zhao, Baozhu,Mao, Jin,Zhou, Qi,Deng, Qianchun,Zheng, Lei,Shi, Jie. 2023

[5]Hollow Hierarchical Porous and Antihydrolytic Spherical Zeolitic Imidazolate Frameworks for Enzyme Encapsulation and Biocatalysis. Zhao, Baozhu,Yang, Haowen,Mao, Jin,Zhou, Qi,Deng, Qianchun,Zheng, Lei,Shi, Jie. 2024

[6]Kinetic-Controlled Synthesis of Walnut-like Core–Shell Magnetic Mesoporous Silica Microspheres for Enhanced Enzyme Loading and Biocatalytic Performance. Zhonglin He,Yuqi Fan,Rongju Zhou,Baozhu Zhao,Xingxing Ding,Jin Mao,Jie Shi. 2025

[7]Preparation of phytosterol esters based on food-grade Pickering emulsion stabilized by lipase-adsorbed zein-hydroxypropyl chitosan nanoparticles. Zhouliang Sun,Yufei Zhang,Huanyu Zheng,Yi Zhang,Yang Li,Mingming Zheng. 2025

作者其他论文 更多>>