数字农科院2.0

A Novel Laboratory Protocol for Pollen Viability Assessment to Inform Biosafety Evaluation of Transgenic Rice

文献类型： 外文期刊

作者： 陈宇枭;;刘彩月;;张晓春;;董玉凤;;王冬梅;;杨江涛;;王志兴;;王旭静

关键词： pollen viability; biosafety policy; rice (Oryza sativa L.)

期刊名称： Agriculture-Basel

ISSN：

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Rice (Oryza sativa L.) is a vital staple crop, and the environmental risk assessment of transgenic varieties is crucial for formulating biosafety policies. Rice pollen grains are spherical, with an average diameter of 40.03 ± 2.75 µm. This study established a standardized protocol for in vitro pollen germination by first optimizing key culture conditions. A single-factor experimental design identified the optimal medium composition as 150 g/L sucrose, 40 mg/L boric acid, 20 mg/L calcium chloride, 10 mg/L monopotassium phosphate, and 10 mg/L magnesium sulfate. The ideal germination temperature was determined to be 31 ± 1 °C, with no germination observed below 16 °C or above 40 °C. Pollen germination rates declined significantly within 5 min post-isolation and ceased completely after 30 min. Building on this optimized protocol, a standardized evaluation method was developed, defining key assessment conditions at temperatures of 25/31/37 °C and post-isolation times of 0/5/15 min. Under these defined conditions, the pollen viability of glyphosate-resistant transgenic rice G2-6 was compared to its non-transgenic recipient ZH11. No significant differences were found at any tested time–temperature combination (p > 0.05). This work establishes a practical and reproducible standard for transgenic rice pollen assessment, offering a scientific basis for evidence-based biosafety regulation and policymaking.

分类号：

  • 相关文献

[1]GLUCAN SYNTHASE-LIKE 5 (GSL5) Plays an Essential Role in Male Fertility by Regulating Callose Metabolism During Microsporogenesis in Rice. Shi, Xiao,Sun, Xuehui,Zhang, Zhiguo,Feng, Dan,Zhang, Qian,Han, Lida,Wu, Jinxia,Lu, Tiegang.

[2]Pollen Semi-Sterility1 Encodes a Kinesin-1-Like Protein Important for Male Meiosis, Anther Dehiscence, and Fertility in Rice. Zhou, Shirong,Wang, Yang,Li, Wanchang,Zhao, Zhigang,Ren, Yulong,Wang, Yong,Jiang, Ling,Liu, Linglong,Wan, Jianmin,Zhou, Shirong,Gu, Suhai,Lin, Qibing,Wang, Dan,Su, Ning,Zhang, Xin,Cheng, Zhijun,Lei, Cailin,Wang, Jiulin,Guo, Xiuping,Wu, Fuqing,Wang, Haiyang,Wan, Jianmin,Ikehashi, Hiroshi.

[3]Pollen viability and stigma receptivity in Lilium during anthesis. He, Guoren,Ming, Jun,Liu, Chun,Yuan, Suxia,He, Guoren,Hu, Fengrong.

[4]Shade Delayed Flowering Phenology and Decreased Reproductive Growth of Medicago sativa L.. Qin, Fengfei,Shen, Yixin,Li, Zhihua,Qu, Hui,Feng, Jinxia,Kong, Lingna,Teri, Gele,Luan, Haoming,Cao, Zhiling. 2022

[5]Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice. Wang Tao,Lou Lijuan,Li Zeyu,Shang Lianguang,Wang Quan. 2021

[6]Pollen grain number and viability contribute to variation in effective ovule number among oilseed rape genotypes. Xiao Guo,Bao Luo Ma,Neil B. McLaughlin,Yajun Gao,Xiaoming Wu,Biyun Chen. 2022

[7]A Novel Laboratory Protocol for Pollen Viability Assessment to Inform Biosafety Evaluation of Transgenic Rice (Oryza sativa L.). Yuxiao Chen,Caiyue Liu,Xiaochun Zhang,Yufeng Dong,Jiangtao Yang,Dongmei Wang,Zhixing Wang,Xujing Wang. 2025

[8]Development and Application of an In Vitro Pollen Viability Assay for Comparative Safety Assessment of Transgenic Alfalfa (Medicago sativa L.). Chen, Yuxiao,Zhang, Xiaochun,Yang, Jiangtao,Guo, Diandian,Wang, Xujing,Wang, Zhixing. 2025

[9]Optimization of grape artificial hybrid pollination technology process and the effect of different male parents on the fruit setting rate. Zhou, Ruijin,Gai, Ruxin,Liao, Wanying,Wu, Qiujie,Cheng, Jiahui,Li, Yongmu,Fan, Xiucai,Li, Guirong. 2025

[10]Cs3, A Ycf54 Domain-Containing Protein, A.ffects Chlorophyll Biosynthesis In R ice (Oryza Sativa L.). Zhang, Yingxin,Cao, Liyong,Zhang, Yingxin,Zeng, Bo,Rani, Mohammad Hasanuzzaman,Cao, Liyong,Cao, Yongrun,Yu, Ning,Liu, Qunen,Cao, Yongrun,Cheng, Shihua,Cheng, Shihua,Chen, Yuyu,Chen, Yuyu,Rani, Mohammad Hasanuzzaman,Zhang, Yue,Yu, Ning,Liu, Qunen,Zhang, Yue. 2019

[11]Lmm24 Encodes Receptor-Like Cytoplasmic Kinase 1.09, Which Regulates Cell D eath And Defense Responses In Rice. Yu，Ning,Chen，Yuyu,Cao，Yongrun,Khan, Shahbaz,Zhan，Xiaodeng,Cheng, Shihua,Cao, Liyong,Zhang，Yue,Zhang，Yingxin,Liu，Qunen. 2019

[12]Stability Of The Metabolite Signature R.esulting From The Ossultr3;3 M utation In Low Phytic Acid Rice (Oryza Sativa L.) Seeds Upon Cross-Breeding. Li, Youfa,Tan, Yuanyuan,Shu, Qingyao,Engel, Karl-Heinz,Gossner, Sophia,Zhou, Chenguang. 2018

[13]Involvement Of Cat In The D.etoxification Of Ht-Induced Ros B urst In Rice Anther And Its Relation To Pollen Fertility. Zhao, Qian,Cheng, Fangmin,Huang, Fudeng,Cheng, Fangmin,Pan, Gang,Du, Xiaoxia,Cao, Zhenzhen,Liu, Jianchao,Zhou, Lujian. 2018

[14]Chlorophyll Composition, Chlorophyll Fluorescence, And G.rain Yield Change In E sl Mutant Rice. Li, Zhaowei,Lin, Weiwei,Li, Zhaowei,Guo, Xiaodong,Pan, Xinfeng. 2018

[15]Chlorophyll Deficient 3, Encoding A P.utative Potassium Efflux Antiporter, A ffects Chloroplast Development Under High Temperature Conditions In Rice (Oryza Sativa L.). Lv, Yusong,Tang, Shaoqing,Wei, Xiangjin,Jiang, Hanwei,Jiang, Hanwei,Sheng, Zhonghua,Hu, Shikai,Shao, Gaoneng,Hu, Peisong,Luo, Rongjian. 2018

[16]Ump Kinase Activity Is Involved I.n Proper Chloroplast Development I n Rice. Dong, Guojun,Wu, Jiahuan,Ma, Xiaohui,Wu, Limin,Yu, Yanchun,Zhang, Yanli,Wang, Fang,Wang, Huizhong,Xiong, Erhui,Chen, Fei,Qian, Qian. 2018

[17]Phytic Acid Contents And Metabolite P.rofiles Of Progenies From C rossing Low Phytic Acid Osmik And Osmrp5 Rice (Oryza Sativa L.) Mutants. Gossner, Sophia,Zhou, Chenguang,Li, Youfa,Engel, Karl:Heinz,Shu, Qingyao,Tan, Yuanyuan,Shu, Qingyao,Tan, Yuanyuan. 2019

[18]Rice Morphogenesis And Chlorophyll Accumulation I.s Regulated By The P rotein Encoded By Nrl3 And Its Interaction With Nal9. Wei, Xiangjin,Cai, Yicong,Wang, Jianlong,Xie, Lihong,Chen, Wei,Chen, Wei,Hu, Peisong,Jiao, Guiai,Tang, Shaoqing,Hu, Peisong,Shao, Gaoneng,Sheng, Zhonghua,Li, Qianlong,Li, Qianlong. 2019

[19]Validation Of Qgs10, A Quantitative T.rait Locus For Grain S ize On The Long Arm Of Chromosome 10 In Rice (Oryza Sativa L.). Wang, Z, Chen, JY, Zhu, YJ, Fan, YY, Zhuang, JY. 2017

[20]Nitrogen Metabolism In Adaptation Of P.hotosynthesis To Water Stress I n Rice Grown Under Different Nitrogen Levels. Zhong, C, Cao, XC, Hu, JJ, Zhu, LF, Zhang, JH, Huang, JL, Jin, QY. 2017

作者其他论文 更多>>