Polymeric hydrogel improves biocontrol effect of Pseudomonas chlororaphis subsp. aureofaciens DA3-5 against Fusarium wilt of cabbage
文献类型: 外文期刊
作者: Du, Jianfeng;Liu, Yiming;Zhao, Xin;Tang, Jiawei;Li, Heng;Wang, Wenlu;Sun, Fuxin;Wu, Limin;Li, Yue;Gao, Qixiong;Lu, Ninghai;Tian, Xueliang;Wang, Chuanjun;Jiao, Yang
作者机构:
关键词: rhizosphere bacteria;Pseudomonas chlororaphis subsp. aureofaciens;cabbage Fusarium wilt;phenazine-1-carboxylic acid;colonization
期刊名称: PEST MANAGEMENT SCIENCE
ISSN: 1526-498X
年卷期: 2025 年
页码:
收录情况: SCIE(2025版)
摘要: BACKGROUND Biological control using rhizobacteria for plant disease management presents a sustainable alternative to conventional chemical fungicides. However, these bacteria demonstrate vulnerability to harsh environmental conditions, resulting in unstable colonization efficiency.RESULTS This study identified Pseudomonas chlororaphis subsp. aureofaciens DA3-5 exhibiting broad-spectrum antimicrobial activity. The DA3-5 fermentation extract (RFE) demonstrated inhibition of mycelial growth in Fusarium oxysporum f. sp. conglutinans (FOC) through disruption of cell wall and membrane function. Analysis revealed that RFE contains phenazine-1-carboxylic acid (PCA) at a concentration of 3.52 mu g mL-1, which effectively inhibits FOC mycelial growth. This study proposes a polymeric hydrogel (PMH) composed of carboxymethyl chitosan, sodium alginate, and calcium chloride for DA3-5 encapsulation.CONCLUSION This hydrogel system enhances both biological control efficiency and rhizosphere colonization effectiveness in cabbage. The developed microbial encapsulation strategy shows promise in protecting sensitive microorganisms under controlled conditions, offering a potential approach for sustainable agriculture that requires further field validation. (c) 2025 Society of Chemical Industry.
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