数字农科院2.0

Genome assembly of the Chinese maize elite inbred line RP125 and its EMS mutant collection provide new resources for maize genetics research and crop improvement

文献类型： 外文期刊

作者： Shujun Nie;Bo Wang;Haiping Ding;Haijian Lin;Li Zhang;Qigui Li;Yujiao Wang;Bin Zhang;Anping Liang;Qi Zheng;Hui Wang;Huayang Lv;Kun Zhu;Minghui Jia;Xiaotong Wang;Jiyuan Du;Runtai Zhao;Zhenzhen Jiang;Caina Xia;Zhenghao Qiao;Xiaohu Li;Boyan Liu;Hongbo Zhu;Rong An;Yucui Li;Qian Jiang;Benfang Chen;Hongkai Zhang;Dening Wang;Changxiao Tang;Yang Yuan;Jie Dai;Jing Zhan;Weiqiang He;Xuebo Wang;Jian Shi;Bin Wang;Min Gong;Xiujing He;Peng Li;Li Huang;Hui Li;Chao Pan;Hong Huang;Guangsheng Yuan;Hai Lan;Yongxin Nie;Xinzheng Li;Xiangyu Zhao;Xiansheng Zhang;Guangtang Pan;Qingyu Wu;Fang Xu;Zhiming Zhang

作者机构：

关键词： elite inbred RP125;EMS mutant collection;genome assembly;kernel mutant;Zea mays

期刊名称： Plant Journal

ISSN： 0960-7412

年卷期： 2021 年 108.0 卷 1.0 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Maize is an important crop worldwide, as well as a valuable model with vast genetic diversity. Accurate genome and annotation information for a wide range of inbred lines would provide valuable resources for crop improvement and pan-genome characterization. In this study, we generated a high-quality de novo genome assembly (contig N50 of 15.43 Mb) of the Chinese elite inbred line RP125 using Nanopore long-read sequencing and Hi-C scaffolding, which yield highly contiguous, chromosome-length scaffolds. Global comparison of the RP125 genome with those of B73, W22, and Mo17 revealed a large number of structural variations. To create new germplasm for maize research and crop improvement, we carried out an EMS mutagenesis screen on RP125. In total, we obtained 5818 independent M2 families, with 946 mutants showing heritable phenotypes. Taking advantage of the high-quality RP125 genome, we successfully cloned 10 mutants from the EMS library, including the novel kernel mutant qk1 (quekou: “missing a small part” in Chinese), which exhibited partial loss of endosperm and a starch accumulation defect. QK1 encodes a predicted metal tolerance protein, which is specifically required for Fe transport. Increased accumulation of Fe and reactive oxygen species as well as ferroptosis-like cell death were detected in qk1 endosperm. Our study provides the community with a high-quality genome sequence and a large collection of mutant germplasm.

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