文献类型： 外文期刊

作者： Jianli Yan; Jiaojiao Ji; Changbin Sun; Wei Xu

关键词： N/A

期刊名称： Science China-Life Sciences

ISSN： 1674-7305

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Several next-generation genomic sequencing (NGS) methods have been developed for mapping DNA modifications, such as BS-seq for 5mC. However, these methods are typically designed to detect a single modification in a single sample per experiment, which significantly limits their utility in constructing comprehensive multiplex and multifactorial modification atlases, and has led to a relative paucity of research on non-5mC modifications in plants. In this study, we developed mDIP-seq (multiplex DNA immunoprecipitation followed by sequencing), a novel DNA modification profiling technique that facilitates high-throughput and cost-effective mapping of multiple DNA modifications. mDIP-seq integrated DNA immunoprecipitation method with the single-stranded DNA (ssDNA) barcoding technique that we previously developed, which enabled strand-specific mapping of DNA modifications.

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