数字农科院2.0

The promotive and repressive effects of exogenous H2O2 on Arabidopsis seed germination and seedling establishment depend on application dose

文献类型： 外文期刊

作者： Yakong Wang;Xiaohong Liu;Xiangyang Sun;Xiaonan Mao;Zhaoye Wang;Jun Peng;Zuoren Yang;Faiza Ali;Zhi Wang;Fuguang Li

作者机构：

关键词： H2O2; Arabidopsis; germination; seedling

期刊名称： Physiologia Plantarum

ISSN： 0031-9317

年卷期： 2025 年 177 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Hydrogen peroxide (H2O2) displays significant and dual effects on seed germination and seedling development, depending on the application dosage. However, the definition of H2O2 thresholds and the mechanisms underlying the dual actions in Arabidopsis seed germination and seedling development are not yet clear. Here, we analyzed the Arabidopsis seed germination profiles in response to different concentrations of exogenous H2O2 and found that 2 mM functions as the key threshold, above this threshold, both seed germination and seedling establishment were gradually inhibited. By RNA-seq analysis and function verification, we identified pathways of abscisic acid (ABA) signalling, seed post-ripening, energy metabolism, ROS homeostasis, and cell wall loosening play positive roles in seed germination and seedling establishment downstream of the H2O2 signalling. Further physio-chemical approaches revealed that exogenous H2O2 affected the accumulation and distribution of O2•- and H2O2 in embryonic tissues by regulating the tissue-specific expression of SDH2-3, RHD2, and PRXs. Collectively, we found that germination rate and aerial growth were positively correlated with endogenous H2O2 content and root length was positively correlated with O2•- accumulation, demonstrating that different ROS signals played specific functions in different tissues and development processes. On the other hand, excessive H2O2 (10 mM) represses these two processes for radicle cell damage caused by oxidation stress. Finally, we put forward the mechanism model of the dual effects of exogenous H2O2 on seed germination and seedling establishment.

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