数字农科院2.0

Investigation of the Migration of Antibiotic Resistance Genes in Soil–Millet System

文献类型： 外文期刊

作者： Zhiping Liu;Ziyuan Guo;Zongyi Wang;Jin Hua;Wenyan Xie;Zhenxing Yang;Liyan He;Xueping Wu;Deli Chen;Huaiping Zhou

作者机构：

关键词： ARGs;MGEs;migration;millet tissues;soil

期刊名称： Agronomy

ISSN：

年卷期： 2025 年 15 卷 12 期

页码：

收录情况： SCIE（2025版）

摘要： The overuse of antibiotics has led to the dissemination of antibiotic resistance genes (ARGs) in agricultural ecosystems, posing a threat to food safety. While current research on ARG transfer in soil–crop systems mainly concerns raw-consumed vegetables like lettuce, its impact on staple crops remains insufficiently studied. This study investigates how organic fertilizer affects ARG dissemination in the soil–millet system. Four fertilization treatments were established: no fertilization (CK), chemical fertilizer only (F), chemical fertilizer combined with manure (FM1), and chemical fertilizer combined with double amount of manure (FM2). Samples were collected from millet rhizosphere soil, roots, stems, leaves, and grains. High-throughput quantitative PCR was employed to investigate the transfer and dissemination of ARGs across the soil–millet system. Results showed that a total of 130 ARGs and 13 mobile genetic elements (MGEs), belonging to 17 gene families, were detected across all samples. The number of unique ARGs was higher in treatments FM1 and FM2 with manure. The accumulated absolute abundances of ARGs, MGEs and gene families all showed an order of FM1 > CK > FM2 > F. Pearson correlation analysis showed a close correlation among ARGs and MGEs. Although organic fertilizer application increased the absolute abundance of ARG-related genes in the rhizosphere soil and millet tissues, posing a potential threat to food safety, the rational strategy employed in the FM1 treatment effectively reduced ARG accumulation in millet leaves and grains. Therefore, this optimized application rate is recommended for millet production.

分类号：

  • 相关文献

[1]Degradation of oxytetracycline in soil by a Pseudomonas strain. Xueqi Guo,Weining Qi,Yao Feng,Zhaojun Li. 2025

[2]Optimizing initial C/N ratio of returned straw with urea mitigates the spread of antibiotic resistance genes in soil. Lijuan Zhao,Zhen Tao,Siyi Li,Taotao Chen,Zhongyang Li,Yuan Liu. 2025

[3]A review of organophosphorus esters in soil: Pollution status, migration, risks, and transformation. Yu Cheng,Xuehao Zheng,Cuihua Hu,Qing Luo,Xingyi Liu,Shoujiang Liu,Peng He,Keke Chang,Fengxia Yang,Yongzhen Ding. 2025

[4]Leaching risk of sulfadiazine in soil: Regulatory role of low-molecular-weight organic acids associated with root exudates. Fuxiang Zhang,Jiaxuan Hong,Qiang Fu,Hongliang Jia,Yi Fan Li,Yongzhen Ding,Xiaoyan Wang,Song Cui,Guorui Liu. 2025

[5]堆形艾美耳球虫巨噬细胞移动抑制因子克隆和表达. 阎晓菲,韩红玉,黄兵,岳城,赵其平,姜连连,董辉,卞庆松,张建哲. 2009

[6]Response of heavy-metal and antibiotic resistance genes and their related microbe in rice paddy irrigated with treated municipal wastewaters. Xiaolong Zheng,Zhengzheng Zhong,Yuan Xu,Xiaoyan Lin,Zhaoyun Cao,Qing Yan. 2023

[7]Multiple Antibiotic-Resistant Bacteria Resistant to Electrochemical Disinfection with Variation of Key Antibiotic Resistance Genes. Jiang, Kaiyang,Li, Hongna,Liu, Wen,Jiang, Yi,Zhang, Zhiguo,Ju, Feng,Song, Tingting,Li, Binxu,Wang, Xuerong,Zhu, Changxiong. 2023

[8]Influence of Dairy Cows Bedding Material on the Microbial Structure and Antibiotic Resistance Genes of Milk. Haoming Wu,Yang Wang,Bingyao Du,Huiying Li,Lei Dong,Haiyan Hu,Lu Meng,Nan Zheng,Jiaqi Wang. 2022

[9]Cropping system exerts stronger influence on antibiotic resistance gene assemblages in greenhouse soils than reclaimed wastewater irrigation. Yuan Liu,Andrew L. Neal,Xiaoxian Zhang,Haiyan Fan,Honglu Liu,Zhongyang Li. 2022

[10]Simultaneous reductions in antibiotic, antibiotic resistance genes and nitrogen loss during bioaugmentation tylosin fermentation dregs co-composting. Bo Zhang,Zhen Guo,Jianhua Qu,Jingdan Zhang,Jie Liu,Yue Tao,Ying Zhang,Muhammad Fahad Sardar,Xiaohu Dai,Huiling Liu. 2022

[11]Deciphering discriminative antibiotic resistance genes and pathogens in agricultural soil following chemical and organic fertilizer. Fengxia Yang,Shizhou Shen,Wenxuan Gao,Yingjun Ma,Bingjun Han,Yongzhen Ding,Xiaolong Wang,Keqiang Zhang. 2022

[12]Fate of antibiotic resistance genes in abandoned swine feedlots in China: seasonal variation. Na Li,Jianguo Chen,Chong Liu,Binxu Li,Changxiong Zhu,Hongna Li. 2021

[13]Effect of wheat crops on the persistence and attenuation of antibiotic resistance genes in soil after swine wastewater application. Wang Y.,Sutton N.B.,Zheng Y.,Dong H.,Rijnaarts H.. 2024

[14]Characterization of heavy metal, antibiotic pollution, and their resistance genes in paddy with secondary municipal-treated wastewater irrigation. Qing Yan,Zhengzheng Zhong,Xiaoyan Li,Zhaoyun Cao,Xiaolong Zheng,Guozhong Feng. 2024

[15]Isolation and characterization of multi-drug-resistant Microbacterium esteraromaticum bacteriophage: assessment of antibacterial efficacy and genomic insights. Cheng, Shenwei,Wang, Hao,Zhang, Keqiang,Liu, Fuyuan,Gao, Xingliang,Du, Lianzhu. 2025

[16]N, P Contribution and soil adaptability of four arbuscular mycorrhizal fungi. Wenke, Liu.

[17]Influence of a heavy rainfall event on the leaching of [C-14]isoproturon and its degradation products in outdoor lysimeters. Doerfler, Ulrike,Cao, Guoyin,Grundmann, Sabine,Schroll, Reiner.

[18]Earthworms enhanced winter oilseed rape (Brassica napus L.) growth and nitrogen uptake. Zhang, Shujie,Chao, Ying,Zhang, Chunlei,Cheng, Jing,Li, Jun,Ma, Ni.

[19]Determination and consideration of flight potential in a laboratory population of true armyworm (Lepidoptera : Noctuidae). Luo, LZ,Johnson, SJ,Hammond, AM,Lopez, JD,Geaghan, JP,Beerwinkle, KR,Westbrook, JK. 2002

[20]Relationship of flight and reproduction in beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), a migrant lacking the oogenesis-flight syndrome. Jiang, Xing Fu,Luo, Li Zhi,Sappington, Thomas W.. 2010

作者其他论文 更多>>