数字农科院2.0

A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus

文献类型： 外文期刊

作者： Qu, Zhaoyang;Tian, Ze;Wei, Liqing;Wang, Chao;Kuang, Lieqiong;Yan, Jiaqi;Wang, Furong;Wang, Nian;Tu, Jinxing;Wang, Xinfa;Wang, Hanzhong;Dun, Xiaoling

作者机构：

关键词： silique length;seed weight;auxin;jasmonic acid;BnaC01.CCT8;CCT complex

期刊名称： PLANT BIOTECHNOLOGY JOURNAL

ISSN： 1467-7644

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Seed weight (SW), which is directly influenced by silique length (SL), is a critical agronomic trait significantly affecting both the quality and yield of rapeseed. In this study, a shorter silique length (ssl) mutant was generated through ethyl methane sulfonate mutagenesis, exhibiting reduced SL and SW compared to the wild type. Utilizing a map-based cloning approach, BnaC01.CCT8, a member of the chaperonin containing T-complex polypeptide-1 (CCT) family, was identified as the gene responsible for restoring the ssl phenotype. A missense mutation from alanine to valine (A507V) in BnaC01.CCT8 was identified as crucial for its functional activity, as evidenced by the genetic complementation of BnaC01.CCT8 and BnaC01.CCT8A507V in the Arabidopsis cct8-2 background. Moreover, overexpression of BnaC01.CCT8 in Brassica napus significantly enhanced SL, SW and seed yield per plant. Conversely, CRISPR/Cas9-mediated bnac01.cct8 knockout lines exhibited reduced SL and SW. Transcriptome analysis and hormone content detection indicated that BnaC01.CCT8 positively regulated SL and SW primarily by modulating auxin and jasmonic acid signalling, thereby affecting the length of epidermal cells in the silique wall. Furthermore, BnaC01.CCT8 interacted with BnaA09.ARF18 (AUXIN-RESPONSE FACTOR 18), contributing to the regulation of SL and SW, while the A507V mutation disrupted this interaction. Haplotype analysis demonstrated that several SNP differences in BnaC01.CCT8 were significantly associated with variations in SL and SW among germplasm resources, revealing superior alleles of BnaC01.CCT8. The identification and functional analysis of BnaC01.CCT8 provide new insights into the mechanisms regulating SL and SW and present a valuable target for the genetic enhancement of rapeseed yield.

分类号：

  • 相关文献

[1]Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed. Liu, Jing,Hua, Wei,Hu, Zhiyong,Yang, Hongli,Zhang, Liang,Deng, Linbin,Sun, Xingchao,Wang, Xinfa,Wang, Hanzhong,Li, Rongjun.

[2]A combined linkage and regional association mapping validation and fine mapping of two major pleiotropic QTLs for seed weight and silique length in rapeseed (Brassica napus L.). Li, Na,Shi, Jiaqin,Wang, Xinfa,Liu, Guihua,Wang, Hanzhong. 2014

[3]Mutation in the LONGIFOLIA1 Gene Resulted in Suppressed Insensitivity of Arabidopsis thaliana proteolysis6 Mutant to Ethylene During Seed Germination. Xu Wang,Ying Luo,Yuan Cao,Yujin Gong,Francoise Corbineau,Yong Xiang. 2025

[4]Genetic mapping of yield traits using RIL population derived from Fuchuan Dahuasheng and ICG6375 of peanut (Arachis hypogaea L.). Chen, Yuning,Ren, Xiaoping,Zheng, Yanli,Zhou, Xiaojing,Huang, Li,Yan, Liying,Jiao, Yongqing,Chen, Weigang,Huang, Shunmou,Wan, Liyun,Lei, Yong,Liao, Boshou,Huai, Dongxin,Wei, Wenhui,Jiang, Huifang.

[5]Genome-wide association study dissects the genetic architecture of seed weight and seed quality in rapeseed (Brassica napus L.). Li, Feng,Chen, Biyun,Xu, Kun,Wu, Jinfeng,Song, Weilin,Liu, Shengyi,Gao, Guizhen,Wang, Nian,Yan, Guixin,Qiao, Jiangwei,Li, Jun,Li, Hao,Xiao, Xin,Zhang, Tianyao,Wu, Xiaoming,Bancroft, Ian,Harper, Andrea L.,Trick, Martin.

[6]Genetic differentiation in relation to seed weights in wild soybean species (Glycine soja Sieb. & Zucc.). Wang, Ke-Jing,Li, Xiang-Hua,Yan, Mao-Fen.

[7]Increasing maize seed weight by enhancing the cytoplasmic ADP-glucose pyrophosphorylase activity in transgenic maize plants. Wang, Zhangying,Chen, Xiaoping,Wang, Jianhua,Liu, Tingsong,Liu, Yan,Zhao, Li,Wang, Guoying. 2007

[8]Genetic dissection of and genomic selection for seed weight, pod length, and pod width in soybean. Chen, Yijie,Xiong, Yajun,Hong, Huilong,Li, Gang,Gao, Jie,Guo, Qingyuan,Sun, Rujian,Ren, Honglei,Zhang, Fan,Wang, Jun,Song, Jian,Qiu, Lijuan. 2023

[9]Genome-wide analysis of the JAZ subfamily of transcription factors and functional verification of BnC08.JAZ1-1 in Brassica napus. Wang, Ying,Li, Na,Zhan, Jiepeng,Wang, Xinfa,Zhou, Xue-Rong,Shi, Jiaqin,Wang, Hanzhong. 2022

[10]Identification and Pyramiding Major QTL Loci for Simultaneously Enhancing Aflatoxin Resistance and Yield Components in Peanut. Gaorui Jin,Nian Liu,Bolun Yu,Yifei Jiang,Huaiyong Luo,Li Huang,Xiaojing Zhou,Liying Yan,Yanping Kang,Dongxin Huai,Yinbing Ding,Yuning Chen,Xin Wang,Huifang Jiang,Yong Lei,Jinxiong Shen,Boshou Liao. 2023

[11]REVIEW FOR GENETIC DISSECTION AND CLONING OF GENES FOR SEED WEIGHT IN OILSEED RAPE. Yao, Y. M.,Solangi, Z. A.,Abro, A. A.,Rattar, T. M.,Ali, U.,Anawar, M.,Ali, A.,Du, D.. 2024

[12]Loss of phytochromobilin synthase activity leads to larger seeds with higher protein content in soybean. Su, Xin,Wang, Hao-Rang,Zhang, Yong,Hong, Hui-Long,Sun, Xu-hong,Wang, Lei,Song, Ji-Ling,Yang, Meng-Ping,Yang, Xing-Yong,Han, Ying-Peng,Qiu, Li-juan. 2025

[13]Heterochrony may underpin the domestication of soybean seed and pod morphology. Yongzhe Gu,Yan Wang,Zhonghui Sun,Shilai Xing,Huihui Gao,Chaoying He. 2025

[14]Seed phenotype and maturity groups as determinants of protein, oil, and fatty acid composition patterns in diverse soybean germplasm. Ahmed M. Abdelghany,Shengrui Zhang,Jing Li,Bin Li,Junming Sun. 2025

[15]Integrating linkage mapping and GWAS reveals novel genetic architecture of seed weight in soybean (Glycine max L.). Zhang, Chunlei,Hong, Huilong,Yuan, Rongqiang,Zhao, Kezhen,Zha, Bire,Lamlom, Sobhi F.,Xi, Xiaoyu,Ren, Honglei,Qiu, Lijuan,Wang, Jiajun. 2026

[16]Jasmonic Acid Signaling Pathway In P.lants. Ruan, Jingjun,Ruan, Jingjun,Khurshid, Muhammad,Zhou, Yuexia,Cheng, Jianping,Zhang, Kaixuan,Zhou, Meiliang,Yan, Jun,Weng, Wenfeng,Yan, Jun,Khurshid, Muhammad. 2019

[17]The response of marigold (Tagetes erecta Linn.) to ozone: impacts on plant growth and leaf physiology. Yang, Ning,Wang, Xiaoke,Zheng, Feixiang,Chen, Yuanyuan,Yang, Ning,Chen, Yuanyuan,Zheng, Feixiang.

[18]TaCPK2-A, a calcium-dependent protein kinase gene that is required for wheat powdery mildew resistance enhances bacterial blight resistance in transgenic rice. Geng, Shuaifeng,Wei, Yuming,Zheng, Youliang,Lan, Xiujin,Geng, Shuaifeng,Li, Aili,Tang, Lichuan,Yin, Lingjie,Wu, Liang,Lei, Cailin,Guo, Xiuping,Zhang, Xin,Mao, Long,Jiang, Guanghuai,Zhai, Wenxue.

[19]Antagonism between phytohormone signalling underlies the variation in disease susceptibility of tomato plants under elevated CO2. Zhang, Shuai,Li, Xin,Sun, Zenghui,Shao, Shujun,Zhou, Yanhong,Xia, Xiaojian,Yu, Jingquan,Shi, Kai,Li, Xin,Yu, Jingquan,Hu, Lingfei,Ye, Meng.

[20]Transgenic expression of a sorghum gene (SbLRR2) encoding a simple extracellular leucine-rich protein enhances resistance against necrotrophic pathogens in Arabidopsis. Zhu, Fu-Yuan,Lo, Clive,Zhu, Fu-Yuan,Zhang, Jianhua,Zhu, Fu-Yuan,Zhang, Jianhua,Li, Lei.

作者其他论文 更多>>