数字农科院2.0

MicroRNA-21 Promotes the Viability, Proliferation and Milk Fat Synthesis of Ovine Mammary Epithelial Cells by Targeting PDCD4

文献类型： 外文期刊

作者： Liyan Hu;Jiqing Wang;Zhiyun Hao;Xian Guo;Mingna Li;Xinmiao Wu;Huimin Zhen;Chunyan Ren;Yuan Zhao;Pan Yang;Xuanyu Wang

作者机构：

关键词： activity;miR-21;ovine mammary epithelial cells;PDCD4;triglyceride

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2025 年 26 卷 4 期

页码：

收录情况： SCIE（2025版）

摘要： MicroRNAs (miRNAs) are short endogenous non-coding RNAs and play important roles in regulating mammary development and activities of ovine mammary epithelial cells (OMECs), which affect the milk yield and milk ingredient contents of ewes. We previously found that miR-21 was highly expressed in ovine mammary tissue, while the regulatory mechanisms of miR-21 underlying mammary development and lactation performance are still unclear. Accordingly, in this study, we investigated the functions of miR-21 in the activities of OMECs, and validated the target relationship of miR-21 with a predicted target gene programmed cell death 4 (PDCD4) by a dual-luciferase reporter assay. Finally, we investigated the regulatory effect of PDCD4 on the viability, proliferation and milk fat synthesis of OMECs. The overexpression of miR-21 significantly increased the viability of OMECs, the number and proportion of Edu-labeled positive OMECs, as well as the contents of triglyceride in OMECs. In fact, miR-21 inhibitor obtained opposite results with miR-21 mimics. The results obtained from the dual luciferase report and RT-qPCR assays confirmed that the seed sequence of miR-21 can complementarily combine with the 3′-untranslated regions (3′-UTR) of PDCD4, and miR-21 decreased the luciferase activity of PDCD4. Meanwhile, miR-21 also reduced the expression of PDCD4. These results indicate that PDCD4 is a target gene of miR-21. It was further found that PDCD4 decreased the viability and triglyceride content of OMECs, and the number and proportion of Edu-labeled positive OMECs. These findings suggest that miR-21 promotes the viability, proliferation and milk fat synthesis of OMECs by down-regulating the expression of PDCD4. The results revealed the regulatory mechanism by which miR-21 affected the activities and milk fat synthesis of OMECs in sheep.

分类号：

  • 相关文献

[1]miR-21靶向猪TSC1和PPP3CA基因的初步鉴定. 彭兴,谢炳坤,唐中林,周荣,敖红,黄生强,李奎. 2013

[2]miR-21与Nocodazole联合作用对小鼠成肌细胞C2C12周期的影响. 刘会杰,敖红,白立景,王志军,邢凯,王楚端. 2015

[3]miR-21在鸭胚胎期胸肌发育中的功能研究. 顾丽红,徐铁山,林哲敏,赵建国,林鹏,杨少雄,林大捷,侯水生. 2019

[4]microRNA-21和TGFBI基因在猪骨骼肌生长发育中的表达分析. 朱师云,谢炳坤,梁如意,李奎,唐中林. 2014

[5]Role of microRNA-21 in the formation of insulin -producing cells from pancreatic progenitor cells. Bai, Chunyu,Li, Xiangchen,Gao, Yuhua,Wang, Kunfu,Fan, Yanan,Zhang, Shuang,Ma, Yuehui,Guan, Weijun.

[6]Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect. Xu, Shuai,Wang, Jufang,Ding, Nan,Hu, Wentao,Zhang, Xurui,Wang, Bing,Hua, Junrui,Wei, Wenjun,Xu, Shuai,Zhu, Qiyun.

[7]Pseudorabies viral replication is inhibited by a novel target of miR-21. Huang, Jing,Fu, Liangliang,Jia, Hao,Zhu, Mengjin,Li, Xinyun,Zhao, Shuhong,Huang, Jing,Ma, Guojian.

[8]Effects of dietary oxidized oil on laying performance, lipid metabolism, and apolipoprotein gene expression in laying hens. Yue, H. Y.,Wang, J.,Qi, X. L.,Ji, F.,Liu, M. F.,Wu, S. G.,Zhang, H. J.,Qi, G. H..

[9]PCK1 expression is correlated with the plasma glucose level in the duck. Chen, L.,Zeng, T.,Li, G. Q.,Tian, Y.,Lu, L. Z.,Li, Q. H.,Liu, R..

[10]Fatty acid and triglyceride molecular species of milk fat fractionated by short-path molecular distillation. Zhu, Huiquan,Wang, Xiaodan,Zhang, Wenyuan,Zhang, Yumeng,Gao, Xixi,Wang, Yunna,Gao, Peng,Pang, Xiaoyang,Zhang, Shuwen,Lv, Jiaping. 2023

[11]Characterization and difference of lipids and metabolites from Jianhe White Xiang and Large White pork by high-performance liquid chromatography–tandem mass spectrometry. Run Zhang,Man Yang,Xinhua Hou,Renda Hou,Ligang Wang,Lijun Shi,Fuping Zhao,Xin Liu,Qingshi Meng,Lixian Wang,Longchao Zhang. 2022

[12]SLC16A7 Promotes Triglyceride Deposition by De Novo Lipogenesis in Chicken Muscle Tissue. Yongli Wang,Lu Liu,Xiaojing Liu,Xiaodong Tan,Yuting Zhu,Na Luo,Guiping Zhao,Huanxian Cui,Jie Wen. 2022

[13]Pre-calving energy density and rumen protected lysine impacted blood metabolites and biomarkers of liver functions in dairy cows during the transition period. Girma Debele Delelesse,Ma Lu,Wang Fang,Callaway Todd R,Bu Dengpan. 2023

[14]Discrimination between the Triglyceride Form and the Ethyl Ester Form of Fish Oil Using Chromatography–Mass Spectrometry. Liu M.,Liu Y.,Wang X.. 2024

[15]Formation and retention of aroma compounds in pigeons roasted by circulating non-fried roast technique by means of UHPLC-HRMS and GC-O-MS. Huan Liu,Jingyu Li,Yuping Zhang,Lin Li,Hansheng Gong,Lixuan Tan,Peng Gao,Pi Li,Jiangtao Xing,Bin Liang,Jianxun Li. 2024

[16]Juvenile hormone inhibits lipogenesis of Spodoptera exigua to response to Bacillus thuringiensis GS57 infection. Bo Gao,Yujie Ji,Dan Zhao,Yitong Yan,Lu Zhang,Han Wu,Yifan Xie,Qiuyu Shi,Yao Wang,Wei Guo. 2024

[17]Key roles of Drosophila flavin-containing monooxygenases in lipid metabolism and longevity. Qi Wei,Yuan Xue,Jianfeng Fan,Jianya Su. 2025

[18]Larval diapause enhances the flight capacity in postdiapause adults of beet webworm, Loxostege sticticalis (Lepidoptera: Pyralidae). Xie, Dian-jie,Lv, Xiao-fei,Zhang, Lei,Cheng, Yun-xia,Jiang, Xing-fu. 2025

[19]Expression and Activity Analysis of Non-Structural Protein 2 (NS2) of Bombyx mori Densovirus Zhenjiang Strain. Zhao Pan,Tang Shun-ming,Guo Xi-jie,Tang Shun-ming,Liu Ting,Qin Guang-xing,Guo Xi-jie. 2010

[20]Synthesis and Antifungal Activity of Novel 1,2,4-Triazole Derivatives Containing 1,2,3-Thiadiazole Moiety. Tan, Cheng-Xia,Weng, Jian-Quan,Liu, Xing-Hai,Zhao, Wei-Guang,Shi, Yan-Xia,Li, Bao-Ju.

作者其他论文 更多>>