The effect of asparagine-13 in porcine epidemic diarrhea virus envelope protein on pathogenicity
文献类型: 外文期刊
作者: Li, Zhiwei;Ma, Zhiqian;Zhao, Xiaojing;Li, Yongqi;Zheng, Congsen;Li, Yang;Guo, Xuyang;Xu, Lele;Zheng, Zifang;Zheng, Haixue;Xiao, Shuqi
作者机构:
关键词: Porcine epidemic diarrhea virus;virulence factor;envelope protein;pro-inflammatory cytokines;reverse genetic system
期刊名称: VETERINARY RESEARCH
ISSN: 0928-4249
年卷期: 2025 年 56 卷 1 期
页码:
收录情况: SCIE(2025版)
摘要: The pathogenesis of porcine epidemic diarrhea virus (PEDV) has not been fully clarified, which seriously hinders the prevention of the disease. The envelope (E) protein of PEDV induces the expression of pro-inflammatory cytokines, but the role of these inflammatory reactions in PEDV pathogenicity is still unknown. In this study, the asparagine at position 13 was found to be crucial to PEDV E protein induced inflammatory response. Exogenously expressing the parent E protein, rather than the E mutant carrying N13A, induces the activation of NF-kappa B and expression of inflammatory factors, including IL-6, IL-8, and TNF-alpha. Compared with the parental rPEDV strain, the recombinant strain rPEDV-EN13A exhibited a significantly lower infectious titer and formed smaller plaques. In addition, rPEDV-EN13A induced lower expression of inflammatory factors in vitro and in vivo. The pathogenicity assay shows that the rPEDV-EN13A strain caused diminished fecal PEDV RNA shedding, delayed death time, and milder histopathological lesions to intestinal villi. Our data provide a unique perspective for exploring the pathogenic mechanism of PEDV and a new target for the development of attenuated PEDV live vaccines.
分类号:
- 相关文献
作者其他论文 更多>>
-
Transcriptome and Phytohormone Analysis Reveal the Regulatory Mechanism of Internode Elongation in New Shoots of Tea Plants (Camellia sinensis)
作者:Zhang, Liping;Shen, Hong;Zhu, Shuixing;Mao, Yihu;Li, Yang;Liu, Haoyang;Jiang, Hongxin;Mohamed, Ahmed Salem;Wang, Peiqiang;Li, Xin
关键词:DELLA;Gibberellins (GAs);Internode;Jasmonate (JA);New shoots;Phytohormones;Tea plant (Camellia sinensis);Transcriptome sequencing
-
Machine Learning-Driven Construction of High-Yielding Cucumber Plant Architectures in Greenhouse Environments
作者:Zhu, Cuifang;Yu, Hongjun;Zhao, Caili;Wu, Hongyang;Wan, Xiaoyang;Lu, Tao;Li, Yang;Jiang, Weijie;Li, Qiang
关键词:cucumber;machine learning;plant architecture;synergistic/antagonistic effects;yield prediction
-
Engineering Metal-Organic Framework Nanosheets as High-Performance Peroxidase Mimics for Visual Detection of African Swine Fever Virus
作者:Deng, Yuanjie;Zhu, Zixiang;Pan, Yangyang;Tian, Hong;Wang, Mingzhu;Wang, Yanxin;Zhou, Chengru;Meng, Lingjie;Zheng, Haixue;Zhao, Xiaoping
关键词:African swine fever virus (ASFV);peroxidase-like;synergistic effect;heterojunction;colorimetricsensor
-
Deciphering the flavor constituents, microbiota and physicochemical properties of Yancaigao, a traditional sour rice paste from Southwest China
作者:Gao, Jinxiao;Li, Yang;Luo, Tingting;Zhang, Yue;Shan, Yimeng;Wang, Aixia;Zhang, Xiya;Wang, Fengzhong;Tong, Li-Tao
关键词:Sour rice paste;Lactic fermentation;Organic acids;Volatiles;Bacterial community
-
Trehalose-6-Phosphate Phosphatase SlTPP1 Adjusts Diurnal Carbohydrate Partitioning in Tomato
作者:Chai, Lin;Wang, Heng;Yu, Hongjun;Li, Hongxue;Yi, Debao;Ikram, Sufian;Cao, Yidan;Zhao, Caili;Lu, Tao;Li, Yang;Jiang, Weijie;Li, Qiang
关键词:diurnal carbohydrate partitioning;Solanum lycopersicum L;stem carbohydrate remobilisation;T6P/SnRK1;transitory starch;trehalose 6-phosphate phosphatase
-
Optimization and analysis of the seed viability monitoring for seven crop species to inhibit fungal infection
作者:Xu, Xinyue;Cheng, Chongyang;Li, Yang;Xin, Xia;Wang, Yingmin;Zhang, Jinmei;Deng, Hui;Deng, Zhijun;Yin, Guangkun
关键词:seed viability monitorin; fungal infection; seven crops; Fusarium; Alternaria; Cladosporium
-
Ferritin and Encapsulin Nanoparticles as Effective Vaccine Delivery Systems: Boosting the Immunogenicity of the African Swine Fever Virus C129R Protein
作者:Zhang, Yue;Ru, Yi;Zhao, Longhe;Hao, Rongzeng;Yang, Yang;Li, Yajun;Zhang, Rong;Jiang, Chenghui;Zheng, Haixue
关键词:nanoparticles;immunogenicity;ferritin;encapsulin;African swine fever virus;targeted lymph nodes;C129R protein