数字农科院2.0

Physiological, biochemical, and transcriptomic analyses revealed enhanced salt tolerance in rice via heterologous expression of Pyropia yezoensis APX gene

文献类型： 外文期刊

作者： Xueli Lu;;Syeda Wajeeha Gillani;;Chen Meng;;Yiqiang Li;;Kexiang Wang;;Zongchang Xu

关键词： Oryza sativa;; Reactive oxygen species (ROS);; Abiotic stress;; Ion homeostasis;; Halophytes

期刊名称： PLANT PHYSIOLOGY AND BIOCHEMISTRY

ISSN： 0981-9428

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Soil salinity represents a significant limitation to global rice production, disrupting ionic balance, inducing oxidative stress, and impairing plant growth. Although antioxidant genes from halophytic seaweed have been recognized to confer salt tolerance, their functional validation in rice remains largely uncharacterized. This study investigated the role of the Pyropia yezoensis ascorbate peroxidase gene (PyAPX), manganese-superoxide dismutase (PyMnSOD), and Kappaphycus alvarezii Na+/H+ antiporter (KaNa+/H+) in conferring salinity tolerance to rice cultivar ZH11 using morphological, physiological assays, transcriptomics, and WGCNA analyses. PyAPXoverexpressing lines exhibited higher germination rate (96.7 %) than wild-type (69 %) under salt stress. At the seedling stage, these lines maintained greater plant height, root length, and higher chlorophyll b content under salt stress treatment. Physiological analyses further revealed reduced malondialdehyde (MDA) accumulation, lower reactive oxygen species (ROS) accumulation, elevated APX and SOD activities, lower Na+ accumulation, and higher K+ retention compared to wild-type. Comparative transcriptome profiling identified 4550 differentially expressed genes in PyAPX lines under salt stress, enriched in pathways related to photosynthesis-antenna proteins and glutathione metabolism. Notably, 23 glutathione-related genes, including glutathione S-transferases (GSTs), and 13 light-harvesting complex (Lhc) genes were up-regulated. Weighted gene co-expression network analysis (WGCNA) revealed modules positively correlated with PyAPX-overexpressing lines, APX activity, K+ content, and plant growth. Hub genes regulating ABA signaling and ion homeostasis, including OsMYB2P-1, OsWNK1, and OsSAP9, were identified as key mediators of PyAPX-driven salt tolerance. Collectively, these results demonstrate that PyAPX enhanced rice salt tolerance by sustaining growth, photosynthesis, antioxidant defense, and ion homeostasis, providing mechanistic insights and supporting its use in developing salt-resilient cultivars.

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