数字农科院2.0

Difference Analysis on Virulence Genes, Biofilms and Antimicrobial Susceptibility of Escherichia coli from Clinical and Subclinical Bovine Mastitis

文献类型： 外文期刊

作者： Jiakun Zuo;Zhaoyang Lv;Liyan Lian;Zihao Wu;Shaodong Fu;Haiyang Zhang;Jing Wu;Zihao Pan;Yong Yu;Wei Chen;Wei Jiang;Huifang Yin;Zhaoguo Chen;Yunpeng Yi;Xiangan Han;Jinfeng Miao

作者机构：

关键词： antimicrobial resistance;biofilm;bovine mastitis;Escherichia coli;MLST;virulence gene

期刊名称： Veterinary Sciences

ISSN： 2306-7381

年卷期： 2025 年 12 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： Escherichia coli (E. coli) has the ability to induce clinical and subclinical mastitis in dairy cows, causing a huge loss for the dairy industry. In this study, 51 subclinical mastitis isolates and 36 clinical mastitis isolates from eight provinces of China between 2019 and 2021 were used to investigate the differences in their biological characteristics. The results showed that B1 (52.9%) and A (39.1%) were the predominant phylogroups; R1 (50.6%) was the predominant lipopolysaccharide (LPS) core type; and 44 STs (ST10 and ST58 were the most sequence-prevalent STs) and 2 new STs (ST14828 and ST14829) were identified; however, no significant difference was observed between the clinical and subclinical group strains. To compare the virulence gene differences between the clinical and subclinical mastitis-related isolates, 18 common virulence genes (including afaE, eaeA, papC, saa, sfa, ompA, aer, irp2, iucD, escV, sepD, east1, estB, stx2e, CNF1, cba, hlyA and traT) were determined using the PCR method. The results showed that the detection rates of traT, irp2 and iucD in clinical mastitis isolates were significantly higher than those in subclinical mastitis isolates (p ˂ 0.05). Meanwhile, subclinical-group E. coli had stronger biofilm formation abilities than the clinical group (p < 0.05) in 78 (89.7%) mastitis-related E. coli that could form biofilms. Furthermore, 87 mastitis-related E. coli showed severe resistance against tetracycline (37.9%), ampicillin (36.8%), streptomycin (34.5%) and cotrimoxazole (28.7%); their most prevalent resistance genes were blaCTX-M (33.3%), tetA (27.6%), sul2 (18.4%) and strB (28.7%). It was noteworthy that the clinical-group strains had a higher resistance against ampicillin and possessed higher amounts of the resistance gene blaCTX-M (p < 0.05) compared to the subclinical group. This study aims to provide references for preventing the E. coli isolates from inducing different types of mastitis.

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