文献类型: 外文期刊
作者: Yuqiang Bai;Tongjing Yan;Fei Fang;Xin Li;Su Wang;Juan Li;Chengli Hou;Dequan Zhang
作者机构:
关键词: Glycolysis;Proteasome;Proteomic;Tenderness;Very fast chilling
期刊名称: Food Science and Human Wellness
ISSN: 2213-4530
年卷期: 2024 年 13 卷 5 期
页码:
收录情况: SCIE(2024版) ; ; 科技核心(2024版)
摘要: The objective of this study was to evaluate the effects of chilling rate on porcine meat quality from the perspective of proteome using data independent acquisition (DIA)-based quantitative proteomic strategy. M. longissimus thoracis et lumborum (n = 9) was assigned randomly to the control group (3.72 °C/h), very fast chilling-I group (VFC-I, 9.31 °C/h) and VFC-II group (14.43 °C/h). The DIA was used to analyze the difference in proteins under different chilling rates. Results showed that tenderness was improved significantly in meat at the chilling rate of 14.43 °C/h. Seventy-nine differential abundant proteins (fold change > 1.5, P < 0.05), including 46 up-regulated and 33 down-regulated proteins, were identified and mainly involved in carbon metabolism, pyruvate metabolism and proteasome pathways. These pathways indicated that VFC delayed cell metabolism and glycolysis by down-regulating the expression of metabolic enzymes. The tenderness was improved by up-regulating the expression of proteasome and m-calpain.
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