数字农科院2.0

Immobilized Burkholderia cepacia lipase and its application in selective enrichment of EPA by hydrolysis of fish oil

文献类型： 外文期刊

作者： Renbin Liu;Pengjin Li;Shiyou Li;Yong Zhuang;Shanhui Chen;Yongsheng Huang;Yudi Zheng;Yan Xiong;Dong Wang;Mingxiong He;Han Qin;Zhirong Yang

作者机构：

关键词： BCL immobiliztion;Eicosapentaenoic acid;Highly selective enrichment;Hydrolysis reaction;Reusability

期刊名称： LWT

ISSN： 0023-6438

年卷期： 2024 年 206 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Eicosapentaenoic acid (EPA), an orally active Omega-3 long-chain polyunsaturated fatty acid, has attracted much attention for its medical and healthcare value. Due to a large amount of polyunsaturated fatty acids (PUFA) including docosahexanoic acid (DHA) in fish oil, the highly specific enrichment of EPA from fish oil is difficult to achieve. In our study, we conducted immobilization studies on Burkholderia cepacia lipase (BCL) and its application in the selective enrichment of EPA in fish oil. BCL was immobilized on resin LX201A by covalent crosslinking, and immobilization conditions including glutaraldehyde concentration, pH, time were optimized. The optimal immobilization conditions were 0.5% glutaraldehyde, 4 h, and pH 8.0. The loading rate of BCL reached to 93.5%, and its activity was up to 68263 U/g under the optimal immobilization conditions. After hydrolysis reaction of fish oil for 15h catalyzed by immobilized BCL, EPA content showed a more increase by 33.2%, while a slight increase by 0.78% in DHA content. Moreover, after recycling use for 10 times, the catalytic efficiency of immobilized BCL remained at 93.0%. These indicated that immobilized BCL possessed good selectivity, reusability and stability, providing great application potential in EPA selective enrichment in fish oil industry.

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