数字农科院2.0

Transcriptome analysis of long non-coding RNA and mRNA Profiles in VSV-infected BHK-21 Cells

文献类型： 外文期刊

作者： Wuweiyi Han;Xiaojuan Fei;Fan Yang;Xintong Sun;Jianshe Yang;Jinxin Qiu;Luhua Zhang;Wenhui Zhang;Guohua Chen;Wei Han;Xiaobo He;Yongsheng Liu;Weike Li

作者机构：

关键词： Differentially expressed genes;LncRNA;mRNA;RNA-Seq;Vesicular stomatitis virus

期刊名称： BMC Genomics

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Background: Vesicular stomatitis virus (VSV) is a typical non-segmented negative-sense RNA virus of the genus Vesiculovirus in the family Rhabdoviridae. VSV can infect a wide range of animals, including humans, with oral blister epithelial lesions. VSV is an excellent model virus with a wide range of applications as a molecular tool, a vaccine vector, and an oncolytic vector. To further understand the interaction between VSV and host cells and to provide a theoretical basis for the application prospects of VSV, we analyzed the expression of host differentially expressed genes (DEGs) during VSV infection using RNA-Seq. Results: Our analyses found a total of 1015 differentially expressed mRNAs and 161 differentially expressed LncRNAs in BHK-21 cells infected with VSV for 24 h compared with controls. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment showed that the differentially expressed lncRNAs and their target genes were mainly concentrated in pathways related to apoptosis, cancer, disease, and immune system activation, including the TNF, P53, MAPK, and NF-kappaB signaling pathways. The differentially expressed lncRNA can modulate immune processes by regulating genes involved in these signaling transmissions. Ten randomly selected DEGs, namely, Il12rb2, F2, Masp2, Mcl1, FGF18, Ripk1, Fas, BMF, POLK, and JAG1, were validated using RT-qPCR. As predicted through RNA-Seq analysis, these DEGs underwent either up- or downregulation, suggesting that they may play key regulatory roles in the pathways mentioned previously. Conclusions: Our study showed that VSV infection alters the host metabolic network and activates immune-related pathways, such as MAPK and TNF. The above findings provide unique insights for further study of the mechanism of VSV–host interactions and, more importantly, provide a theoretical basis for VSV as an excellent vaccine carrier.

分类号：

  • 相关文献

[1]Temporal transcriptomic changes in long non-coding RNAs and messenger RNAs involved in the host immune and metabolic response during Toxoplasma gondii lytic cycle. Wang, Sha-Sha,Zhou, Chun-Xue,Elsheikha, Hany M.,He, Jun-Jun,Zou, Feng-Cai,Zheng, Wen-Bin,Zhu, Xing-Quan,Zhao, Guang-Hui. 2022

[2]牛体内囊胚全转录组模式解析. 王晶晶,张培培,郝海生,杜卫华,庞云渭,权国波,赵善江,邹惠影,朱化彬,赵学明. 2020

[3]RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis. Yue Xu,Wen Bin Zheng,Hao Yu Li,Lang Cai,Yang Zou,Shi Chen Xie,Xing Quan Zhu,Hany M. Elsheikha. 2022

[4]Transcriptome Analysis Revealed Long Non-Coding RNAs Associated with mRNAs in Sheep Thyroid Gland under Different Photoperiods. Wang W.,He X.,Di R.,Wang X.,Chu M.. 2022

[5]Expression profiles of oviductal mRNAs and lncRNAs in the follicular phase and luteal phase of sheep (Ovis aries) with 2 fecundity gene (FecB) genotypes. Chen, Weihao,Li, Zhifeng,Zhong, Rongzhen,Sun, Wei,Chu, Mingxing. 2023

[6]Oviduct Transcriptomic Reveals the Regulation of mRNAs and lncRNAs Related to Goat Prolificacy in the Luteal Phase. Sun Z.,Hong Q.,Liu Y.,Ren C.,He X.,Jiang Y.,Ouyang Y.,Chu M.,Zhang Z.. 2022

[7]Supplementation with Milk-Derived Extracellular Vesicles Shapes the Gut Microbiota and Regulates the Transcriptomic Landscape in Experimental Colitis. Chunmei Du,Kun Wang,Yiguang Zhao,Xuemei Nan,Ruipeng Chen,Suyu Quan,Benhai Xiong. 2022

[8]Transcriptome analysis reveals a lncRNA-miRNA-mRNA regulatory network in OsRpp30-mediated disease resistance in rice. Minghua Li,Wei Li,Meixia Zhao,Zhiqiang Li,Guo Liang Wang,Wende Liu,Chun Liang. 2023

[9]Hypothalamus Transcriptome Reveals Key lncRNAs and mRNAs Associated with Fecundity in Goats. Yingshi Wei,Caiye Zhu,Xiaoyun He,Mingxing Chu. 2025

[10]Genome-wide transcriptome analysis in the ovaries of two goats identifies differentially expressed genes related to fecundity. Miao, Xiangyang,Luo, Qingmiao,Qin, Xiaoyu.

[11]Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity. Miao, Xiangyang,Qingmiao Luo Xiaoyu Qin. 2015

[12]Comparative transcriptome analysis of different nitrogen responses in low-nitrogen sensitive and tolerant maize genotypes. Qing guo DU,Juan YANG,Shah SYED MUHAMMAD SADIQ,Rong xin YANG,Jing juan YU,Wen xue LI. 2021

[13]The comparative transcriptome analysis of two green super rice genotypes with varying tolerance to salt stress. Zahra, Nageen,Uzair, Muhammad,Zaid, Imdad Ullah,Attia, Kotb A.,Inam, Safeena,Fiaz, Sajid,Abdallah, Rizk M.,Naeem, Muhammad Kashif,Farooq, Umer,Rehman, Nazia,Ali, Ghulam Muhammad,Xu, Jianlong,Li, Zhikang,Khan, Muhammad Ramzan. 2024

[14]RNA-Seq Reveals Pathways Responsible for Meat Quality Characteristic Differences between Two Yunnan Indigenous Chicken Breeds and Commercial Broilers. Yong Liu,Xia Zhang,Kun Wang,Qihua Li,Shixiong Yan,Hongmei Shi,Lixian Liu,Shuangmin Liang,Min Yang,Zhengchang Su,Changrong Ge,Junjing Jia,Zhiqiang Xu,Tengfei Dou. 2024

[15]Transcriptome analysis of Crandell Rees Feline Kidney (CRFK) cells infected with Feline calicivirus strain 023 (FCV 023). Ruiming Zhang,Hongwei Zhu,Guangrong Zhao,Xiu Xue,Xin Yu,Yang Liu,Jiayu Yu,Linlin Jiang,Jianlong Zhang,Xingxiao Zhang. 2025

[16]Discovery of novel long non-coding RNAs induced by subgroup J avian leukosis virus infection in chicken. Qiu, Lingling,Li, Zhiteng,Chang, Guobin,Bi, Yulin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[17]Identification and Characterization of Long Non-coding RNA in Tomato Roots Under Salt Stress. Li N.,Wang Z.,Wang B.,Wang J.,Xu R.,Yang T.,Huang S.,Wang H.,Yu Q.. 2022

[18]RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton. Xianyan Zou,Faiza Ali,Shuangxia Jin,Fuguang Li,Zhi Wang. 2022

[19]A Comprehensive Analysis of the Myocardial Transcriptome in ZBED6-Knockout Bama Xiang Pigs. Shengnan Wang,Wenjie Tian,Dengke Pan,Ling Liu,Cheng Xu,Yuehui Ma,Dandan Wang,Lin Jiang. 2022

[20]Profiling and Functional Analysis of long non-coding RNAs in yak healthy and atretic follicles. Yao Y.,Meng Z.,Li W.,Xu Y.,Wang Y.,Suolang S.,Xi G.,Cao L.,Guo M.. 2022

作者其他论文 更多>>