数字农科院2.0

Transcriptome Reveals the Key Genes Related to the Metabolism of Volatile Sulfur-Containing Compounds in Lentinula edodes Mycelium

文献类型： 外文期刊

作者： Zheng Li;Fei Pan;Wen Huang;Shuangshuang Gao;Xi Feng;Meijie Chang;Lianfu Chen;Yinbing Bian;Wenli Tian;Ying Liu

作者机构：

关键词： KEGG;Lentinula edodes;molecular simulation;mycelium;RNA-seq;sulfur compounds

期刊名称： Foods

ISSN： 2304-8158

年卷期： 2024 年 13 卷 14 期

页码：

收录情况： SCIE（2024版）

摘要： Lentinula edodes (L. edodes) is a globally popular edible mushroom because of its characteristic sulfur-containing flavor compounds. However, the formation of the volatile sulfur-containing compounds in the mycelium of L. edodes has not been studied. We found that there were also sulfur-containing aroma compounds in the mycelium of L. edodes, and the content and composition varied at different stages of mycelial growth and development. The γ-glutamyl-transpeptidase (GGT) and cysteine sulfoxide lyase (C-S lyase) related to the generation of sulfur compounds showed the highest activities in the 15-day sample. Candidate genes for the metabolism of volatile sulfur compounds in mycelium were screened using transcriptome analysis, including encoding the GGT enzyme, C-S lyase, fatty acid oxidase, HSP20, and P450 genes. The expression patterns of Leggt3 and Leccsl3 genes were consistent with the measured activities of GGT and C-S lyase during the cultivation of mycelium and molecular dynamics simulations showed that they could stably bind to the substrate. Our findings provide insights into the formation of sulfur-containing flavor compounds in L. edodes. The mycelium of L. edodes is suggested for use as material for the production of sulfur-containing flavor compounds.

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