数字农科院2.0

A transposon insertion in the 5′ UTR of OsPT1 reprograms its expression pattern and promotes cadmium accumulation in rice grains

文献类型： 外文期刊

作者： Peng, Shasha;Wang, Dan;Liu, Jinling;Jiang, Su;Xu, Yuchen;Deng, Yufei;Zhou, Xiaolong;Hu, Fangzhi;Liu, Zhuo;Peng, Ye;Ao, Hejun;Xiao, Yinghui;Wang, Jiurong;Zhao, Junliang;Liu, Bin;Yi, Keke;Bai, Lianyang;Wang, Guo-Liang;Kang, Houxiang

作者机构：

关键词： rice;cadmium accumulation;loci associated with grain cadmium content;transporter;GWAS;minia-ture inverted-repeat transposable element

期刊名称： PLANT COMMUNICATIONS

ISSN： 2590-3462

年卷期： 2025 年 7 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度）

摘要： Cadmium (Cd) accumulation in rice grains presents a serious risk to human health; however, the mechanisms underlying this process remain incompletely understood. In this study, a genome-wide association analysis identified 29 loci associated with grain Cd content (LAGCCs). Among these, one of the most strongly associated loci, LAGCC4, contains the transporter gene OsPT1, whose haplotypes show a strong correlation with Cd content in rice grains. A transposon, H-MITE, inserts into the 5 ' untranslated region (UTR) of OsPT1, altering its expression pattern and leading to increased Cd accumulation. Furthermore, we identified the transcription factor OsbHLH35, which specifically binds to the OsPT1H-MITE promoter to regulate its transcription in response to Cd stress. Targeted knockout of either OsPT1H-MITE or OsbHLH35 via CRISPR-Cas9 gene editing significantly reduced grain Cd content, with reductions ranging from 61.7% to 80.6%. This study reveals a previously unrecognized mechanism contributing to high Cd accumulation in rice and identifies genetic targets for breeding rice varieties with reduced Cd content.

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