数字农科院2.0

An efficient CRISPR/Cas9-mediated editing of phytoene desaturase in hexaploid sweetpotato

文献类型： 外文期刊

作者： Tang, Wei;Ma, Meng;Song, Weihan;Kou, Meng;Wang, Xin;Yan, Hui;Li, Chen;Zhang, Aicen;Gao, Tianqi;Gao, Runfei;Zhang, Yungang;Li, Qiang

作者机构：

关键词： CRISPR/Cas9;Sweetpotato;Gene editing;Phytoene desaturase;Hi-TOM

期刊名称： PLANT SCIENCE

ISSN： 0168-9452

年卷期： 2025 年 364 卷

页码：

收录情况： SCIE（2025版）

摘要： CRISPR/Cas9-mediated gene editing has emerged as a pivotal tool for functional genomics and crop improvement. For the first time, we applied CRISPR/Cas9-mediated editing to the IbPDS gene in the purple-fleshed cultivar 'XZS-8', achieving mutation efficiencies up to 98.18 %. Loss-of-function mutations in IbPDS induced visually discernible albino phenotypes. Hi-TOM sequencing confirmed deletion mutations within the target locus, with editing efficiencies ranging from 17.77 % to 65.90 % (gRNA1) and 87.87-98.18 % (gRNA2). Knockout lines showed significant reductions in chlorophyll a and b content, confirming functional disruption of IbPDS. Collectively, our results demonstrate efficient CRISPR/Cas9-mediated genome editing for generating mutants in the hexaploid sweetpotato.

分类号：

  • 相关文献

[1]The Potential of CRISPR/Cas9 Gene Editing as a Treatment Strategy for Inherited Diseases. Sameh A. Abdelnour,Long Xie,Abdallah A. Hassanin,Erwei Zuo,Yangqing Lu. 2021

[2]Large DNA fragment deletion in lncRNA77580 regulates neighboring gene expression in soybean (Glycine max). Fengjuan Niu,Qiyan Jiang,Xianjun Sun,Zheng Hu,Lixia Wang,Hui Zhang. 2021

[3]Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes. Hui Tao,Xuetao Shi,Feng He,Dan Wang,Ning Xiao,Hong Fang,Ruyi Wang,Fan Zhang,Min Wang,Aihong Li,Xionglun Liu,Guo Liang Wang,Yuese Ning. 2021

[4]The Potential of CRISPR/Cas9 Gene Editing as a Treatment Strategy for Inherited Diseases. Sameh A. Abdelnour,Long Xie,Abdallah A. Hassanin,Erwei Zuo,Yangqing Lu. 2022

[5]Application of CRISPR/Cas9 for Rapid Genome Editing of Pseudorabies Virus and Bovine Herpesvirus-1. Yu W.,Liu J.,Liu Y.,Forlenza M.,Chen H.. 2024

[6]Physiological and Molecular Mechanisms of Lepidopteran Insects: Genomic Insights and Applications of Genome Editing for Future Research. Dongsheng Niu,Qing Zhao,Linbo Xu,Kejian Lin. 2024

[7]CRISPR/Cas9-mediated mutations of GS3 and GW5 positively regulate grain size and grain width in rice (Oryza sativa indica). Aqib Zeb,Amir Sohail,Shengjia Tang,Zhonghua Sheng,Peisong Hu. 2025

[8]Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice. Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Qian, Qian,Sun, Lei,Zhang, Yan,Lu, Qingtao,Lu, Congming,Han, Bin,Chen, Fan.

[9]In planta haploid induction by genome editing of DMP in the model legume Medicago truncatula. Wang N., Xia X., Jiang T., Li L., Zhang P., Niu L., Cheng H., Wang K., Lin H.. 2023

[10]Multiplex precision gene editing by a surrogate prime editor in rice. Li H., Zhu Z., Li S., Li J., Yan L., Zhang C., Ma Y., Xia L.. 2022

[11]High Throughput Sequencing Identifies Chilling R.esponsive Genes In Sweetpotato ( Ipomoea Batatas Lam.) During Storage. Li, Zongyun,Jiang, Jiaojiao,Han, Yonghua,Han, Yonghua,Xie, Zeyi,Zhang, Baohong,Zhou, Zhilin,Yu, Jingjing,Jiang, Jiaojiao,Li, Hongmin,Zhou, Zhilin,Ma, Daifu,Xie, Zeyi,Yu, Jingjing,Li, Zongyun,Tang, Zhonghou,Tang, Zhonghou,Li, Hongmin,Ma, Daifu. 2019

[12]A Novel Glutathione S-Transferase Gene F.rom Sweetpotato, Ibgstf4, Is I nvolved In Anthocyanin Sequestration. Kou, Meng,Kou, Meng,Tang, Wei,Su, Zai-xing,Yan, Hui,Arisha, Mohamed Hamed,Chen, Xiao-guang,Li, Qiang,Li, Zong-yun,Ma, Dai-fu,Zhang, Yun-gang,Li, Qiang,Ma, Dai-fu,Wang, Xin,Liu, Ya-ju,Liu, Ya-ju. 2019

[13]Development Of Snp Markers Using R.na-Seq Technology And Tetra-Primer A rms-Pcr In Sweetpotato. Wang Xin,Tang Wei,Ma Dai-fu,Zhang Yun-gang,Xu Jia-lei,Wang Xin,Li Qiang,Kou Meng,Kou Meng,Ma Dai-fu,Liu Ya-ju,Li Qiang. 2017

[14]Expression of Arabidopsis NDPK2 increases antioxidant enzyme activities and enhances tolerance to multiple environmental stresses in transgenic sweetpotato plants. Kim, Yun-Hee,Lim, Soon,Yang, Kyoung-Sil,Kim, Cha Young,Lee, Haeng-Soon,Kwak, Sang-Soo,Kwon, Suk-Yoon,Wang, Xin,Zhou, Zhilin,Ma, Daifu,Yun, Dae-Jin,Yun, Dae-Jin.

[15]Chilling And Heat Stress-Induced Physiological Changes And Microrna-Related Mechanism In Sweetpotato (Ipomoea Batatas L.). Yu, Jingjing,Yu, Jingjing,Yu, Jingjing,Li, Zongyun,Li, Zongyun,Zhang, Baohong,Li, Hongmin,Han, Yonghua,Tang, Zhonghou,Dong, Tingting,Su, Dan,Han, Yonghua,Su, Dan,Dong, Tingting,Li, Hongmin,Yang, Dongjing,Yang, Dongjing,Tang, Zhonghou. 2020

[16]Selection of Parents for Breeding Edible Varieties of Sweetpotato with High Carotene Content. Ma Dai-fu,Li Qiang,Li Xiu-ying,Li Hong-min,Tang Zhong-hou,Hu Ling,Cao Qing-he,Xie Yi-ping,Wang Xin. 2009

[17]Survey of sweetpotato viruses in China. Xie, Y. -P.,Xing, J. -Y.,Li, X. -Y.,Wang, X.,Sun, H. -J.,Zhao, Y. -Q.,Zhang, C. H. -L.,Ma, D. -F.,Xie, Y. -P.,Xing, J. -Y.,Li, X. -Y.,Wang, X.,Sun, H. -J.,Zhao, Y. -Q.,Zhang, C. H. -L.,Ma, D. -F.. 2013

[18]Generation Of A New Thermo-Sensitive G.enic Male Sterile Rice L ine By Targeted Mutagenesis Of Tms5 Gene Through Crispr/Cas9 System. Wang, Wei,Wei Xiangjin,Sheng Zhonghua,Barman, Hirendra Nath,Jiao Guiai,Barman, Hirendra Nath,Cai Yicong,Zhong, Min,Hu Peisong,Tang Shaoqing,Wu Yawen,Fiaz, Sajid. 2019

[19]Time-ordering japonica/geng genomes analysis indicates the importance of large structural variants in rice breeding. Wang, Yu,Li, Fengcheng,Zhang, Fan,Wu, Lian,Xu, Na,Sun, Qi,Chen, Hao,Yu, Zhiwen,Lu, Jiahao,Jiang, Kai,Wang, Xiaoche,Wen, Siyu,Zhou, Yao,Zhao, Hui,Jiang, Qian,Wang, Jiahong,Jia, Ruizong,Sun, Jian,Tang, Liang,Xu, Hai,Hu, Wei,Xu, Zhengjin. 2022

[20]CRISPR-Cas System, a Possible “Savior” of Rice Threatened by Climate Change: An Updated Review. Nabeel Shaheen,Shakeel Ahmad,Salem S. Alghamdi,Hafiz Mamoon Rehman,Muhammad Arshad Javed,Javaria Tabassum,Gaoneng Shao. 2023

作者其他论文 更多>>