数字农科院2.0

Suppression of Nodule Formation by RNAi Knock-Down of Bax inhibitor-1a in Lotus japonicus

文献类型： 外文期刊

作者： Fuxiao Jin;Danxia Ke;Lu Lu;Qianqian Hu;Chanjuan Zhang;Chao Li;Wanwan Liang;Songli Yuan;Haifeng Chen

作者机构：

关键词： Bax inhibitor-1;infection thread;nodulation;nodule formation;RNA interference;root nodule symbiosis

期刊名称： Genes

ISSN： 2073-4425

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background/Objectives: The balanced regulation of innate immunity plays essential roles in rhizobial infection and the establishment and maintenance of symbiosis. The evolutionarily conserved cell death suppressor Bax inhibitor-1 plays dual roles in nodule symbiosis, providing a valuable clue in balancing immunity and symbiosis, while it remains largely unexplored in the legume Lotus japonicus. Methods/Results: In the present report, the BI-1 gene family of L. japonicus was identified and characterized. We identified 6 BI-1 genes that translate into peptides containing 240–255 amino acids with different structural characteristics and isoelectric points. We performed phylogenetic analyses and detected evolutionary conservation and divergence among BI-1 proteins from L. japonicus, Glycine max, Medicago truncatula, Arabidopsis thaliana, and Oryza sativa. Expression profiles among different roots indicated that the inoculation of MAFF303099 significantly increased the expression of most of the L. japonicus BI-1 family genes. We down-regulated the transcripts of LjBI-1a by RNA interference and observed that LjBI-1a promotes nodulation and nodule formation. Conclusions: These discoveries shed light on the functions of BI-1 genes in L. japonicus, and simultaneously emphasize the potential application of LjBI-1a in enhancing the symbiotic nitrogen fixation ability of legumes.

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