数字农科院2.0

Identification and utilization of a novel large-effect QTL and linked markers for enhancing protein concentration in peanut

文献类型： 外文期刊

作者： Mingjun Wang;Jianbin Guo;Gaorui Jin;Taihua Yang;Weigang Chen;Yuning Chen;Li Huang;Huaiyong Luo;Xiaojing Zhou;Boshou Liao;Huifang Jiang;Nian Liu;Yong Lei

作者机构：

关键词： peanut; protein content; QTL mapping

期刊名称： Theoretical and Applied Genetics

ISSN： 0040-5752

年卷期： 2025 年 138 卷 9 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Key message: A novel large-effect QTL qPCB03 for protein concentration was identified, and a KASP marker linked to qPCB03 was developed and validated to enhance protein concentration by 1.23 to 1.57 percentage points in the RIL populations. Abstract : Peanut is a vital source of protein for humans, playing a key role in maintaining a steady protein supply. In this study, the high-protein cultivar Zhonghua6 (29.14 ± 1.69%) was crossed with Xuhua13 (24.55 ± 1.84%) to construct a recombinant inbred line (RIL) population. The protein concentration of the RIL population exhibited significant variation, ranging from 21.05 to 30.28%. To discover genomic regions associated with protein concentration, four libraries were constructed (two parents and two extreme bulks) for bulked segregant sequencing (BSA-seq). The results revealed significant associations between protein concentration and the genomic regions on chromosomes A07, A10, B03, and B10. Through linkage analysis, nine QTLs (quantitative trait loci) for protein concentration were identified, among which the large-effect and stable QTL qPCB03 on chromosome B03 (125.92–127.94 Mb) explained 11.03–12.86% of the phenotypic variation. This QTL (qPCB03), simultaneously identified by both BSA-Seq and linkage mapping, had not been documented in prior studies. Within the ~ 2 Mb interval of qPCB03, a total of 349 genomic variants were discovered, including six single nucleotide polymorphisms (SNPs) that resulted in nonsynonymous mutations in six genes. Based on genomic variants of the target region, KASP markers were developed and validated in 147 peanut varieties exhibiting variable seed protein concentration. According to the marker-assisted selection, favorable genotypes could potentially enhance protein concentration by 1.23–1.57 percentage points in the RIL populations. The identification of stable loci and the development of markers facilitate marker-assisted breeding in peanuts, while the discovery of candidate genes lays the groundwork for the fine mapping of key genes regulating protein concentration.

分类号：

  • 相关文献

[1]Using Hyperspectral Analysis As A P.otential High Throughput Phenotyping T ool In Gwas For Protein Content Of Rice Quality. Zhao, Nan,Zhu, Yueming,Zheng, Hongkun,Zhu, Yueming,Li, Xiaolong,He, Yong,Liu, Fei,Shu, Qingyao,Liu, Fei,He, Yong,El-Manawy, Ahmed Islam,Weng, Haiyong,El-Manawy, Ahmed Islam,Cen, Haiyan,Fu, Haowei,Sun, Dawei,Cen, Haiyan,Cen, Haiyan,Abdalla, Alwaseela,He, Yong,Abdalla, Alwaseela,Tang, Juan,Sun, Dawei,Wan, Liang,Weng, Haiyong,Wan, Liang. 2019

[2]A Combined Linkage And Gwas Analysis Identifies Qtls Linked To Soybean Seed Protein And Oil Content. Sun, Shi,Yuan, Shan,Hou, Wensheng,Wu, Cunxiang,Han, Tianfu,Wang, Liwei,Wu, Tingting,Zhang, Tengfei,Jiang, Bingjun,Zhen, Caixin,Hou, Wensheng. 2019

[3]Genetic Dissection Of Root Morphological T.raits As Related To P otassium Use Efficiency In Rapeseed Under Two Contrasting Potassium Levels By Hydroponics. Shi, Jiaqin,Wang, Jie,Wang, Xinfa,Dun, Xiaoling,Wang, Hanzhong,Liu, Huiping. 2019

[4]Identification And Qtl Mapping Of Z.550, A Rice Backcrossed I nbred Line With Increased Grains Per Panicle. Yang Zheng-lin,Wang Shi-ming,Cui Guo-qing,Ling Ying-hua,Ma Fu-ying,Xia Sai-sai,Zhang Chang-wei,He Guang-hua,Li Yun-feng,Wang Hui,Zhao Fang-ming. 2019

[5]Identification Of Qtls Associated With C.admium Concentration In Rice G rains. Hu Pei-song,Chen Wei,Hu Da-wei,Hu Pei-song,Jiao Gui-ai,Li Qian-long,Shao Gao-neng,Sheng Zhong-hua,Tang Shao-qing,Xie Li-hong,Wang Jian-long,Wei Xiang-jin. 2018

[6]A High-Density Genetic Map Of A.n Allohexaploid Brassica Doubled H aploid Population Reveals Quantitative Trait Loci For Pollen Viability And Fertility. Li, Lan,Yan, Guijun,Meng, Jinling,Zhang, Kangni,Yin, Yuling,Gill, Rafaqat A.,Li, Lan,Zhang, Kangni,Yang, Su,Yan, Guijun,Yang, Su,Gill, Rafaqat A.,Chen, Sheng,Zhou, Weijun,Cowling, Wallace A.,Cowling, Wallace A.,Yang, Su,Yang, Su,Chen, Sheng,Zhou, Weijun,Cowling, Wallace A.. 2018

[7]Detection Of Quantitative Trait Loci U.nderlying Yield-Related Traits In S hiitake Culinary-Medicinal Mushroom, Lentinus Edodes (Agaricomycetes). Zhou, Yan,Li, Lei,Gong, Wen-bing,Kwan, Hoi-shan,Gong, Wen-bing,Cheung, Man-kit,Xiao, Yang,Bian, Yin-bing. 2018

[8]High-Density Genetic Map Construction And Q.tl Mapping For Fiber S trength On Chr24 Across Multiple Environments In A Ccri70 Recombinant Inbred Lines Population. Fan, Liqiang,Ge, Qun,Liu, Aiying,Lei, Kang,Liu, Ruixian,Li, Junwen,Gong, Juwu,Yuan, Youlu,Duan, Li,Shang, Haihong,Shi, Yuzhen,Zou, Xianyan,Gong, Wankui,Wang, Yanling,Fan, Senmiao,Gong, Juwu,Jiang, Xiao,Zhen, Zhang,Duan, Li,Zhang, Qi. 2018

[9]An Integrated Analysis Of Qtl M.apping And Rna Sequencing P rovides Further Insights And Promising Candidates For Pod Number Variation In Rapeseed (Brassica Napus L.). Yang, Yuhua,Wang, Xinfa,Zhan, Jiepeng,Ye, Jiang,Wang, Hanzhong,Liu, Guihua,Chen, Bo,Shi, Jiaqin,Luo, Meizhong. 2017

[10]Genetic Dissection Of Lint Yield A.nd Fiber Quality Traits O f G. Hirsutum In G. Barbadense Background. Zhang, Tianzhen,Zhu, Xiefei,Si, Zhanfeng,Cao, Zhibin,Chen, Hong. 2017

[11]Qtl Mapping Of Grain Zn A.nd Fe Concentrations In T wo Hexaploid Wheat Ril Populations With Ample Transgressive Segregation. Crespo-Herrera, LA, Govindan, V, Stangoulis, J, Hao, YF, Singh, RP. 2017

[12]Genetic Dissection Of Flowering Time I.n Brassica Rapa Responses T o Temperature And Photoperiod. Xiao, D, Shen, HR, Zhao, JJ, Wei, YP, Liu, DR, Hou, XL, Bonnema, G. 2019

[13]Genetic Mapping Of Quantitative Trait L.oci For The Stigma E xsertion Rate In Rice (Oryza Sativa L.). Rahman, MH, Zhang, YX, Sun, LP, Zhang, KQ, Rahman, MS, Wu, WX, Zhan, XD, Cao, LY, Cheng, SH. 2017

[14]Inheritance Of Steroidal Glycoalkaloids In P.otato Tuber Flesh. Peng, Z, Wang, P, Tang, D, Shang, Y, Li, CH, Huang, SW, Zhang, CZ. 2019

[15]Qtl Mapping And Candidate Gene M.ining For Soybean Seed W eight Per Plant. Yu, M, Liu, ZX, Jiang, SS, Xu, N, Chen, QS, Qi, ZM, Lv, WH. 2018

[16]Qtl Mapping In Three Connected P.opulations Reveals A Set O f Consensus Genomic Regions For Low Temperature Germination Ability In Zea Mays L.. Li, XH, Wang, GH, Fu, JJ, Li, L, Jia, GY, Ren, LS, Lubberstedt, T, Wang, GY, Wang, JH, Gu, RL. 2018

[17]Identification and Verification of Quantitative Trait Loci Affecting Milling Yield of Rice. 朱玉君,,朱安东,,樊叶杨,,庄杰云. 2020

[18]Construction And Integration Of Genetic Linkage Maps From Three Multi-Parent Advanced Generation Inter-Cross Populations In Rice. 屈平平, 史金卉, 王建康, 徐建龙, 张鲁燕. 2020

[19]High-Density Linkage Map Construction And M.apping Of Salt-Tolerant Qtls A t Seedling Stage In Upland Cotton Using Genotyping By Sequencing (Gbs). Diouf, L, Pan, ZE, He, SP, Gong, WF, Jia, YH, Magwanga, RO, Romy, KRE, Rashid, HO, Kirungu, JN, Du, XM. 2017

[20]Identification Of A Novel Locus, Bph38(T), Conferring Resistance To Brown Planthopper (Nilaparvata Lugens Stal.) Using Early Backcross Population In Rice (Oryza Sativa L.). Balachiranjeevi, CH, Prahalada, GD, Mahender, A, Jamaloddin, M, Sevilla, MAL, Marfori-Nazarea, CM, Vinarao, R, Sushanto, U, Baehaki, SE, Li, ZK, Ali, J. 2019

作者其他论文 更多>>