Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization
文献类型: 外文期刊
作者: Feifei Guan;Xiaoqian Tian;Ruohan Zhang;Yan Zhang;Ningfeng Wu;Jilu Sun;Honglian Zhang;Tao Tu;Huiying Luo;Bin Yao;Jian Tian;Huoqing Huang
作者机构:
关键词: Chitin;Chitinase;Endo-activity;Functional oligosaccharides;Mutants
期刊名称: Bioresources and Bioprocessing
ISSN: 2197-4365
年卷期: 2024 年 11 卷 1 期
页码:
收录情况: SCIE(2024版) ; ; EI(2024版)
摘要: Thermophilic endo-chitinases are essential for production of highly polymerized chitooligosaccharides, which are advantageous for plant immunity, animal nutrition and health. However, thermophilic endo-chitinases are scarce and the transformation from exo- to endo-activity of chitinases is still a challenging problem. In this study, to enhance the endo-activity of the thermophilic chitinase Chi304, we proposed two approaches for rational design based on comprehensive structural and evolutionary analyses. Four effective single-point mutants were identified among 28 designed mutations. The ratio of (GlcNAc)3 to (GlcNAc)2 quantity (DP3/2) in the hydrolysates of the four single-point mutants undertaking colloidal chitin degradation were 1.89, 1.65, 1.24, and 1.38 times that of Chi304, respectively. When combining to double-point mutants, the DP3/2 proportions produced by F79A/W140R, F79A/M264L, F79A/W272R, and M264L/W272R were 2.06, 1.67, 1.82, and 1.86 times that of Chi304 and all four double-point mutants exhibited enhanced endo-activity. When applied to produce chitooligosaccharides (DP ≥ 3), F79A/W140R accumulated the most (GlcNAc)4, while M264L/W272R was the best to produce (GlcNAc)3, which was 2.28 times that of Chi304. The two mutants had exposed shallower substrate-binding pockets and stronger binding abilities to shape the substrate. Overall, this research offers a practical approach to altering the cutting pattern of a chitinase to generate functional chitooligosaccharides. Graphical abstract: (Figure presented.)
分类号:
- 相关文献
作者其他论文 更多>>
-
Partial protective efficacy of the current licensed Japanese encephalitis live vaccine against the emerging genotype I Japanese encephalitis virus isolated from sheep
作者:Hailong Zhang;Yan Zhang;Dan Li;Jiayang Zheng;Junjie Zhang;Zongjie Li;Ke Liu;Beibei Li;Donghua Shao;Yafeng Qiu;Zhiyong Ma;Jianchao Wei;Juxiang Liu
关键词:Japanese encephalitis virus;neutralizing antibodies;protective efficacy;SA14-14-2 vaccine;sheep
-
Fructose metabolism in Entner-Doudoroff pathway-deficient Cupriavidus necator H16 depends on the Calvin shunt
作者:Lijuan Ding;Lin Cui;Yuheng Wang;Beau Dronsella;Xu Liu;Huiying Luo;Huoqing Huang;Xiaoyun Su;Yingguo Bai;Jie Zhang;Tao Tu;Xing Qin;Yuan Wang;Yaru Wang;Bin Yao;Xianli Xue;Gang Guo;Xiaolu Wang
关键词:Calvin shunt;Cupriavidus necator;Entner-Doudoroff pathway;Phosphoglycolate salvage pathway
-
An evolutionarily ancient transcription factor drives spore morphogenesis in mushroom-forming fungi
作者:Zhihao Hou;Zsolt Merényi;Yashu Yang;Yan Zhang;Árpád Csernetics;Balázs Bálint;Botond Hegedüs;Csenge Földi;Hongli Wu;Zsolt Kristóffy;Edit Ábrahám;Nikolett Miklovics;Máté Virágh;Xiao Bin Liu;Nikolett Zsibrita;Zoltán Lipinszki;Ildikó Karcagi;Wei Gao;László G. Nagy
关键词:
-
Alleviating Clostridium perfringens-Induced Intestinal Lesions in Chickens Using the Xylanase CbXyn10C and Its Binary Cocktail with a Protease
作者:Wenjing Zhang;Zhenzhen Hao;Daoxin Yang;Wangli Ji;Kairui Guo;Xianhua Sun;Shuai Wang;Shuyan Yang;Jianshuang Ma;Tong Wang;Huiying Luo;Bin Yao;Meiling Zhang;Yuan Wang;Huoqing Huang;Xiaoyun Su
关键词:broiler chickens;Clostridium perfringens;gut microbiota modulation;necrotic enteritis;protease;xylanase
-
Juvenile Hormone and Ecdysteroids Facilitate the Adult Reproduction Through the Methoprene-Tolerant Gene and Ecdysone Receptor Gene in the Female Spodoptera frugiperda
作者:Yan Zhang;Kui Ting Ding;Nian Wan Yang;Zhi Chuang Lv;Zhen Ying Wang;Yong Jun Zhang;Wan Xue Liu;Jian Yang Guo
关键词:EcR;hormone;Met;reproduction;RNA interference;Spodoptera frugiperda
-
Composite Probiotics Improve Gut Health and Enhance Tryptophan Metabolism in Nursery Piglets During Liquid Feeding
作者:Man Du;Qifan Zhang;Yutian Shen;Jie Fu;Yizhen Wang;Bin Yao;Zeqing Lu
关键词:gut microbiota;liquid feeding;probiotic;tryptophan metabolism
-
Two carboxyl/choline esterase (CCE) genes, TuCCE11 and TuCCE34, are related to abamectin resistance in Tetranychus urticae Koch
作者:Yan Zhang;Ke Wang;Dandan Xu;Chunyue Zhu;Zhaojiang Guo;Wen Xie;Xin Yang;Qingjun Wu;Youjun Zhang;Shaoli Wang
关键词:Abamectin;Carboxyl/cholinesterases;Resistance;Tetranychus urticae