数字农科院2.0

PGAM5 suppresses porcine epidemic diarrhea virus replication via selective autophagy-mediated degradation of the viral nucleocapsid protein

文献类型： 外文期刊

作者： Yang, Xinyu;Qin, Wenzhen;Tong, Wu;Yu, Hai;Zheng, Hao;Tong, Guangzhi;Zhang, Yu;Kong, Ning;Shan, Tongling

作者机构：

关键词： PGAM5;Replication;Selective autophagy;Nucleocapsid Protein

期刊名称： VETERINARY MICROBIOLOGY

ISSN： 0378-1135

年卷期： 2025 年 311 卷

页码：

收录情况： SCIE（2025版）

摘要： PGAM5, a mitochondrial phosphatase involved in mitophagy and apoptosis, has lately been identified as a potential antiviral factor. Nonetheless, the mechanism whereby PGAM5 suppresses viral replication via the selective autophagy pathway remains unclear. In the present work, we discovered that PGAM5 inhibits PEDV replication through selective autophagy. After PEDV infected LLC-PK1 cells, PGAM5 protein levels decreased while mRNA levels increased. Furthermore, PGAM5 overexpression suppressed replication of PEDV in LLC-PK1 and Vero cells, while knocking down PGAM5 increased viral replication. Crucially, PGAM5 interacts with the nucleocapsid (N) protein, recruiting cargo receptor p62 along with E3 ubiquitin ligase STUB1 for degrading N protein, thus suppressing PEDV replication. Our findings clarify PGAM5's antiviral mechanism and provide insights into developing PEDV control strategies.

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