数字农科院2.0

Amphiphilicity and thermostability modification of dipeptidyl peptidase III from aspergillus terreus HNGD-TM15 capable of simultaneously degrading aflatoxin B1 and zearalenone

文献类型： 外文期刊

作者： Zhang, Hongxin;Zhang, Shujie;Ma, Weibin;Yang, Yuhui;Li, Xiao;Xie, Yanli

作者机构：

关键词： dipeptidyl peptidase III;aflatoxin B 1;zearalenone;self-assembled amphiphilic peptides;amphiphilicity;thermostability

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2025 年 496 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Aflatoxin B1 (AFB1) and Zearalenone (ZEN) are significant mycotoxins that pose serious risks to food safety and human health. This study characterized a dipeptidyl peptidase III (AsDPP III) from Aspergillus terreus HNGDTM15, which degraded 98.25 % of AFB1 (5.0 mu g/mL) and 96.68 % of ZEN (10 mu g/mL). Additionally, a selfassembling amphiphilic peptide S1 was fused to the N-terminus of AsDPP III, resulting in the amphiphilic enzyme S1-AsDPP III, which demonstrated 83.36 % and 70.08 % degradation rates for AFB1 and ZEN in vegetable oil, respectively. Moreover, S1-AsDPP III exhibits enhanced thermostability, with its half-life at 80 degrees C extended 1.85-fold, and superior storage stability after seven weeks. Furthermore, the degradation products were identified as AFQ1 for AFB1 and DHZEN for ZEN. Zebrafish exposure assays revealed that the degradation products exhibited markedly reduced toxicity compared to the parent mycotoxins. These findings provide a promising strategy for developing efficient detoxifying enzymes for food and feed applications.

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