数字农科院2.0

Volatile organic compounds from Meyerozyma guilliermondii JY19 trigger apoptosis-like cell death in Botrytis cinerea and control postharvest gray mold of ginseng berries

文献类型： 外文期刊

作者： Guanzhong Ding;Fan Zhou;Peng Mu;Lidan Zhang;Yue Zhang;Yidong Zheng;Wenwen Jiang;Ning Liu

作者机构：

关键词： Biofumigation mechanism;HS-SPME-GC-MS profiling;MAPK signaling pathway;Oxidative stress;Pyrazine-based antifungal agents;Yeast-derived metabolites

期刊名称： Postharvest Biology and Technology

ISSN： 0925-5214

年卷期： 2026 年 235 卷

页码：

收录情况： SCIE（2025版）

摘要： Gray mold caused by Botrytis cinerea severely compromises the postharvest value of ginseng berries. We assessed the endophytic yeast Meyerozyma guilliermondii JY19 as a green control and delineated a VOC-mediated mode of action. JY19 inhibited B. cinerea in vitro in diffusible-metabolite, cell-free supernatant, and sealed double-plate VOC assays. Scanning electron microscopy revealed hyphal surface collapse and fissures. VOC exposure induced reactive oxygen species and programmed cell death in conidia based on DCFH-DA staining, Annexin V–FITC/PI imaging, and flow cytometry. HS-SPME–GC–MS profiling identified a characteristic VOC repertoire; 3,5-diethyl-2-methylpyrazine, 4-methyl-2-pentanol, and trans-ocimenol showed dose-dependent inhibition with IC₅₀ values of 9.5, 23.1, and 26.7 μL L⁻¹, respectively, whereas 1-hydroxy-2-propanone was inactive. RNA-seq showed concerted repression of nutrient-transport systems and xenobiotic detoxification (including multiple cytochrome P450s), together with attenuation of sphingolipid and glycosphingolipid pathways and MAPK signaling; oxidative phosphorylation was upregulated, consistent with metabolic compensation under VOC. In vivo, JY19 VOCs delayed disease onset and reduced lesion area by 79.3 %, 77.3 %, and 39.3 % on days 1, 2, and 3, respectively, thereby limiting decay under high humidity. These multi-level data support a mode-of-action model in which JY19 VOCs compromise membrane integrity and nutrient acquisition, trigger ROS-driven programmed cell death, and constrain fungal growth and virulence. To our knowledge, this is the first integrated in vitro, cytological, transcriptomic, and in vivo evaluation of VOC-based gray mold control on ginseng berries, positioning JY19 and its dominant VOCs as residue-lean biofumigation leads for postharvest management.

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