数字农科院2.0

Development of a Recombinase Polymerase Amplification Fluorescence Assay for the Detection of Canine Adenovirus 2

文献类型： 外文期刊

作者： Li Xiao;Mengdi Zhang;Zhige Tian;Ye Ge;Tongyuan Zhang;Li Yi;Feng Cong

作者机构：

关键词： canine adenovirus 2;fluorescence;limit of detection;real-time;recombinase polymerase amplification

期刊名称： Frontiers in Veterinary Science

ISSN： 2297-1769

年卷期： 2021 年 8 卷

页码：

收录情况： JCR（2021版）

摘要： Canine adenovirus type 2 (CAdV-2) is often found in co-infections with other pathogens causing canine infectious respiratory disease (CIRD). Rapid, efficient, and convenient pathogen detection is the best approach for early confirmatory diagnosis. In this study, we developed and evaluated a rapid real-time recombinase polymerase amplification (RPA) assay for detection of canine adenovirus 2 (CAV), which can detect CAV within 15 min at 39°C. The detection limit that assay was 214 copies/μl DNA molecules per reaction. The specificity was indicated by a lack of cross-reaction with canine distemper virus (CDV), canine coronavirus (CCV), and canine parvovirus (CPV). Field and clinical applicability of this assay were evaluated using 86 field samples. The coincidence rate of the detection results for clinical samples between CAV-RPA and qPCR was 97.7%. In summary, the real-time CAV-RPA analysis provides an efficient, rapid and sensitive detection method for CAV.

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