数字农科院2.0

A novel allele encoding 7‐hydroxymethyl chlorophyll a reductase confers bacterial blight resistance in rice

文献类型： 外文期刊

作者： Marie Gorette Kampire;Ringki Kuinamei Sanglou;Huimei Wang;Bello Babatunde Kazeem;Jian Li Wu;Xiaobo Zhang

作者机构：

关键词： Bacterial blight;Defense response;OsHCAR;Rice

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2021 年 22.0 卷 14.0 期

页码：

收录情况： JCR（2021版）

摘要： Rice spotted leaf mutants are helpful to investigate programmed cell death (PCD) and defense response pathways in plants. Using a map‐based cloning strategy, we characterized novel rice spotted leaf mutation splHM143 that encodes a 7‐hydroxymethyl chlorophyll a reductase (OsHCAR). The wild‐type (WT) allele could rescue the mutant phenotype, as evidenced by complementation analysis. OsHCAR was constitutively expressed at all rice tissues tested and its expression products localized to chloroplasts. The mutant exhibited PCD and leaf senescence with increased H2O2 (hydrogen peroxide) accumulation, increased of ROS (reactive oxygen species) scavenging enzymes activities and TUNEL (terminal deoxyribonucleotidyl transferase‐mediated dUTP nick‐end labeling) ‐positive nuclei, upregulation of PCD related genes, decreased chlorophyll (Chl) contents, downregulation of photosynthesis‐related genes, and upregulation of senescence‐associated genes. Besides, the mutant exhibited enhanced bacterial blight resistance with significant upregulation of defense response genes. Knockout lines of OsHCAR exhibited spotted leaf phenotype, cell death, leaf senescence, and showed increased resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) coupled with upregulation of five pathogenesis‐related marker genes. The overexpression of OsHCAR resulted in increased susceptibility to Xoo with decreased expression of pathogenesis‐related marker genes. Altogether, our findings revealed that OsHCAR is involved in regulating cell death and defense response against bacterial blight pathogen in rice.

分类号：

  • 相关文献

[1]ASSESSMENT OF A FUNCTIONAL MARKER FOR BACTERIAL BLIGHT RESISTANCE GENE LOCUS Xa 27IN RICE. Yu, P.,Yuan, X. P.,Wang, C. H.,Xu, Q.,Feng, Y.,Yu, H. Y.,Wang, Y. P.,Wei, X. H.,Wang, X. M..

[2]SEQUENCE VARIATIONS AND HAPLOTYPES OF THE BACTERIAL BLIGHT RESISTANCE GENE xa13 IN RICE. Yu, P.,Yuan, X. P.,Wang, C. H.,Xu, Q.,Feng, Y.,Yu, H. Y.,Wang, Y. P.,Wei, X. H.,Wang, X. M..

[3]Xa39, a novel dominant gene conferring broad-spectrum resistance to Xanthomonas oryzae pv. oryzae in rice. Zhang, F.,Zhuo, D. -L.,Zhang, F.,Huang, L. -Y.,Wang, W. -S.,Xu, J. -L.,Li, Z. -K.,Zhou, Y. -L.,Xu, J. -L.,Li, Z. -K.,Zhou, Y. -L.,Vera Cruz, C..

[4]Genetic Overlap in the Quantitative Resistance of Rice at the Seedling and Adult Stages to Xanthomonas oryzae pv. oryzae. Zhou, Yong-Li,Xie, Xue-Wen,Xu, Mei-Rong,Zang, Jin-Ping,Zhu, Ling-Hua,Xu, Jian-Long,Li, Zhi-Kang,Li, Zhi-Kang.

[5]Development of markers for identification and maker-assisted breeding of Xa7 gene in rice (Oryza sativa L.). Pengcheng Liu,Le Mei,Lumei He,Yiling Xu,Yiting Zhang,Dali Zeng,Xiaoming Zhang,Qian Qian,Xifeng Chen,Bojun Ma. 2021

[6]Pyramiding blast, bacterial blight and brown planthopper resistance genes in rice restorer lines. JI Zhi-juan (2), Yang Shu-dong +, ZENG Yu-xiang , LIANG Yan , YANG Chang-deng *, QIAN Qian (2)*. 2016

[7]Rice MPK17 Plays a Negative Role in the Xa21-Mediated Resistance Against Xanthomonas oryzae pv. oryzae. Zheng Zhu,Tianxingzi Wang,Jinping Lan,Jinjiao Ma,Haiqing Xu,Zexi Yang,Yalu Guo,Yue Chen,Jianshuo Zhang,Shijuan Dou,Ming Yang,Liyun Li,Guozhen Liu. 2022

[8]Introgression of bacterial blight resistance genes in the rice cultivar ciherang: Response against xanthomonas oryzae pv. oryzae in the f6 generation. Priya Lal Biswas,Ujjal Kumar Nath,Sharmistha Ghosal,Gayatri Goswami,Md Shalim Uddin,Omar M. Ali,Arafat Abdel Hamed Abdel Latef,Alison M. Laing,Yong Ming Gao,Akbar Hossain. 2021

[9]A KNOX II transcription factor suppresses the NLR immune receptor BRG8-mediated immunity in rice. Xu, Siliang,Wei, Xinghua,Yang, Qinqin,Hu, Dongxiu,Zhang, Yuanyuan,Yuan, Xiaoping,Kang, Fengyu,Wu, Zhaozhong,Yan, Zhiqin,Luo, Xueqin,Sun, Yanfei,Wang, Shan,Feng, Yue,Xu, Qun,Zhang, Mengchen,Yang, Yaolong. 2024

[10]OsMKK1 is a novel element that positively regulates the Xa21-mediated resistance response to Xanthomonas oryzae pv. oryzae in rice. Yang Z.X.,Zhu Z.,Guo Y.,Lan J.,Zhang J.,Chen S.,Dou S.,Yang M.,Li L.,Liu G.. 2024

[11]A Novel Single Base Mutation in OsSPL42 Leads to the Formation of Leaf Lesions in Rice. Panpan Li,Huihui Shang,Xia Xu,Junyi Gong,Jian Li Wu,Xiaobo Zhang. 2024

[12]OsHUB1 and OsHUB2 interact with SPIN6 and form homo- and hetero-dimers in rice. Zhang, Hao,Liu, Jinling,He, Feng,Wang, Zhilong,Wang, Guo-Liang,Zhang, Hao,Liu, Jinling,He, Feng,Ning, Yuese,Wang, Guo-Liang,Wang, Guo-Liang. 2015

[13]OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Liu, XQ,Bai, XQ,Qian, Q,Wang, XH,Chen, MS,Chu, CC. 2005

[14]Identification and characterization of short crown root 8, a temperature-sensitive mutant associated with crown root development in rice. Peng Hu,Yi Wen,Yueying Wang,Hao Wu,Junge Wang,Kaixiong Wu,Bingze Chai,Lixin Zhu,Guangheng Zhang,Zhenyu Gao,Deyong Ren,Li Zhu,Longbiao Guo,Dali Zeng,Jing Xu,Song Yan,Qian Qian,Yuchun Rao,Jiang Hu. 2021

[15]Identification and characterization of short crown root 8, a temperature-sensitive mutant associated with crown root development in rice. Peng Hu,Yi Wen,Yueying Wang,Hao Wu,Junge Wang,Kaixiong Wu,Bingze Chai,Lixin Zhu,Guangheng Zhang,Zhenyu Gao,Deyong Ren,Li Zhu,Longbiao Guo,Dali Zeng,Jing Xu,Song Yan,Qian Qian,Yuchun Rao,Jiang Hu. 2021

[16]水稻淡褐斑叶突变体lbsll的遗传分析与基因定位. 奉保华,杨杨,施勇烽,林璐,陈洁,黄奇娜,魏彦林,HeiLEUNG,吴建利. 2012

[17]OsBTF3转基因水稻对病原茵侵染的反应和防卫基因的表达分析. 陈华民,吴茂森,何晨阳. 2012

[18]Identification of QTLs underlying resistance to a virulent strain of Xanthomonas oryzae pv. oryzae in rice cultivar DV85. Wang, CM,Su, CC,Zhai, HQ,Wan, JM. 2005

[19]Detecting major QTL associated with resistance to bacterial blight using a set of rice reciprocal introgression lines with high density SNP markers. Zhang, Fan,Xu, Meirong,Wang, Wensheng,Xu, Jianlong,Zhou, Yongli,Li, Zhikang,Xie, Xuewen,Li, Zhikang,Xu, Jianlong,Zhou, Yongli.

[20]RFLP mapping and race specificity of bacterial blight resistance genes (QTLs) in rice. Luo, LJ,Mei, HW,Zhao, XH,Zhong, DB,Wang, YP,Yu, XQ,Ying, CS,Li, ZK,Paterson, AH,Wang, DL,Tabien, RE,Zhu, L,Stansel, JW.

作者其他论文 更多>>