数字农科院2.0

Improving the catalytic efficiency of a highly thermostable phenylalanine ammonia-lyase from Nostoc sp. ATCC 53789 and its application in producing low L-phenylalanine protein

文献类型： 外文期刊

作者： Han, Xue;Wang, Yulu;Wei, Xue;Yu, Haiyan;Liu, Shujun;Tan, Huishuang;Xin, Fengjiao

作者机构：

关键词： Phenylketonuria;Phenylalanine ammonia-lyase;Rational design;Protein hydrolysates;Low L-Phe protein preparation

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2025 年 501 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Daily dietary supplementation with low L-phenylalanine (L-Phe) protein is crucial for phenylketonuria (PKU) patients. Phenylalanine ammonia-lyase (PAL) catalyzes L-Phe deamination to trans-cinnamic acid, offering a sustainable enzymatic strategy for generating low L-Phe proteins. Here, a novel PAL from Nostoc sp. ATCC 53789 (NoPAL) was identified, purified, and characterized. NoPAL exhibited strict specificity for L-Phe and retained 90 % residual activity after 2 h at 70 degrees C, marking a 40 % improvement over the commercial Anabaena variabilis PAL. Structure-guided engineering using AlphaFold3 predictions yielded four beneficial mutants (S73N, F84Y, V90R and E95V), showing 1.5-2.3 fold higher catalytic efficiency without compromising stability. These mutants efficiently deaminated L-Phe in various protein hydrolysates, and S73N performed best, achieving 87 %, 95 % and 86.7 % conversion rates for casein acid hydrolysate, whey protein hydrolysate and rice protein hydrolysate, respectively. These findings indicate NoPAL S73N has great potential for producing specialized low L-Phe proteins tailored for PKU patients.

分类号：

  • 相关文献

[1]Argininosuccinate synthetase gene is silenced by CpG methylation in children with phenylketonuria. Li, Li,Shao, GenZe,Lin, Ming,Jin, ChunMei,Ye, LiTao,Wang, LiDe. 2013

[2]Phenylalanine ammonia-lyase and cinnamate 4-hydroxylase genes' response to HHO in Eupatorium adenophorum. Guo, Huiming,Cheng, Hongmei,Wan, Fanghao. 2012

[3]N6-Methyladenosine Modification Regulates Prunus Necrotic Ringspot Virus Infection in Cucumis sativus. Dong, Zhenfei,Liu, Na,Zhang, Zhixiang,Wang, Hongqing,Li, Shifang,Zhan, Binhui. 2025

[4]Phenylalanine Ammonia-Lyase as a Key Enzyme in Tea Plant Resistance to Herbivory. Wang, Ran,Chai, Zhichao,Yu, Yongchen,Qian, Xiaona,Wang, Jia,Sun, Xiaoling,Zhang, Xin. 2025

[5]Evaluation of free radical-scavenging activities of sweet potato protein and its hydrolysates as affected by single and combination of enzyme systems. Zhang, Miao,Mu, Tai-Hua,Sun, Min-Jie,Wang, Yan-Bo. 2012

[6]Comparative study of antioxidant and flavour characteristics of Maillard reaction products from five types of protein hydrolysates. Habinshuti, Ildephonse,Zhang, Miao,Sun, Hong-Nan,Mu, Tai-Hua. 2022

[7]The Effects of Amino Acids, Phenols and Protein Hydrolysates as Biostimulants on Sustainable Crop Production and Alleviated Stress. Shahrajabian M.H., Cheng Q., Sun W.. 2022

[8]Amino Acids Biostimulants and Protein Hydrolysates in Agricultural Sciences. Sun W.,Shahrajabian M.H.,Kuang Y.,Wang N.. 2024

[9]Computer-aided rational design of novel EBF analogues with an aromatic ring. Wang, Shanshan,Du, Shaoqing,Qin, Yaoguo,Duan, Hongxia,Yang, Xinling,Sun, Yufeng. 2016

[10]Engineering the Active Site Pocket to Enhance the Catalytic Efficiency of a Novel Feruloyl Esterase Derived From Human Intestinal Bacteria Dorea formicigenerans. Shen, Yang,Wang, Yulu,Wei, Xue,Wen, Boting,Liu, Shujun,Tan, Huishuang,Zhang, Jingjian,Shao, Shuli,Xin, Fengjiao. 2022

[11]Surface charge modifications modulate glucose oxidase pH-activity profiles for efficient gluconic acid production. Yan Y.,刘晓青.,Jiang X.,张伟.,Wang Y.,Wang Y.,Zhang Y.,Luo H.,Yao B.,Huang H.,Tu T.. 2022

[12]Improving the Activity and Stability of Serine Protease ThAPT3 by Alleviating Self-Cleavage and Its Application in Deproteinization of Shrimp Shells. Xiao Wang,Tao Dong,Qiao Zhou,Lige Tong,Jie Zheng,Xing Qin,Xiaolu Wang,Yaru Wang,Bin Yao,Huoqing Huang,Huiying Luo. 2023

[13]Enhancing the thermostability of transglutaminase from Streptomyces mobaraensis based on the rational design of a disulfide bond. Hongjing Wang,Haiqing Chen,Qingbin Li,Fan Yu,Yaru Yan,Shuang Liu,Jian Tian,Jianxin Tan. 2022

[14]Rational Design for Broadened Substrate Specificity and Enhanced Activity of a Novel Acetyl Xylan Esterase from Bacteroides thetaiotaomicron. Luyao Wang,Xue Han,Yulu Wang,Xue Wei,Shujun Liu,Shuli Shao,Shaoqing Yang,Lichao Sun,Fengjiao Xin. 2021

[15]Design, Synthesis, and Biological Activity of Novel Benzo[d][1,3]dioxole-6-benzamide Derivatives: Multichitinase Inhibitors as Potential Insect Growth Regulator Candidates. Jin, Xiaoyu,Sun, Tengda,Guo, Bingbo,Cui, Jialin,Ling, Yun,Zhang, Li,Yang, Qing,Chen, Wei,Yang, Xinling. 2023

[16]Achieving thermostability of a phytase with resistance up to 100 °C. Tao Tu,Qian Wang,Ruyue Dong,Xiaoqing Liu,Leena Penttinen,Nina Hakulinen,Jian Tian,Wei Zhang,Yaru Wang,Huiying Luo,Bin Yao,Huoqing Huang. 2024

作者其他论文 更多>>