数字农科院2.0

Identification and primary application of hybridomas cell secreting monoclonal antibodies against mink (Neovison vison) interferon-gamma

文献类型： 外文期刊

作者： Hailing Zhang;Shasha Zhang;Sining Fan;Lei Zhang;Bo Hu;Xue Bai;Dongliang Zhang;Rongguang Lu;Jianjun Zhao;Shizhen Lian;Bing Gao;Xijun Yan;Shiying Lu;Yanzhu Zhu

作者机构：

关键词： ELISA method;Hybridoma clones;Interferon-gamma;Lymphocyte;Mink;Monoclonal antibody

期刊名称： Cytokine

ISSN： 1096-0023

年卷期： 2022 年 150 卷

页码：

收录情况： JCR（2021版）

摘要： Due to their susceptibility to several human viruses, the mink has been proposed as potential animal models for the study of human viral infections. However, there are no specific monoclonal antibody (mAbs) currently available for the detection of mink-specific interferon-gamma (miIFN-γ). The BALB/c mice were immunized intraperitoneally with purified recombinant miIFN-γ protein. The splenocytes were obtained and fused with murine myeloma cells. Five of 24 hybridoma clones were obtained to produce mAbs steadily with the strongest affinity to recombinant miIFN-γ protein. The isotype of the 31A, 31B and 31G were lgG 2b. The isotype of 44 and 46 were lgG 2a and 1. All five mAbs were κ light chains. Western blotting and indirect ELISA method showed that 5 mAbs were positive to miIFN-γ. Immunofluorescence showed that 2 mAbs (44 and 46) had a positive reaction to miIFN-γ. The hybridoma clone 46 had the highest sensitivity for the detection of miIFN-γ. Most importantly, our primary sandwich ELISA system (mAbs 46 and polyclonal antiserum) detected endogenous IFN-γ in mink lymphocytes infected with canine distemper virus (CDV). We have thus developed a novel mAbs could recognize miIFN-γ, and have demonstrated the first ELISA-based measurement of IFN-γ in lymphocyte of the mink.

分类号：

  • 相关文献

[1]Cloning and expression of mink (Neovison vison) interferon-gamma gene and development of an antiviral assay. Zhang, Hailing,Zhao, Jianjun,Bai, Xue,Zhang, Lei,Hu, Bo,Liu, Hao,Xu, Shujuan,Yan, Xijun,Zhang, Hailing,Yan, Xijun,Zhang, Dongliang,Fan, Sining.

[2]Establishment of ELISA method for canine adenovirus type 1. Ben Wang,Jinfeng Xu,Hui Zhang,Shizhen Lian,Yichang Duan,Hongling Zhang,Wei Hou,Baishuang Yin,Yanzhu Zhu. 2024

[3]In vivo transcription of bovine leukemia virus and bovine immunodeficiency-like virus. Wu, DL,Murakami, K,Morooka, A,Jin, H,Inoshima, Y,Sentsui, H. 2003

[4]Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes. Zhao, Y. H.,Zhang, Z. B.,Zhao, C. Q.,Zhang, Y.,Wang, Y. F.,Zhu, Z. Q.,Guan, W. J.. 2015

[5]Boron Affects Immune Function Through Modulation of Splenic T Lymphocyte Subsets, Cytokine Secretion, and Lymphocyte Proliferation and Apoptosis in Rats. Jin, Erhui,Li, Shenghe,Ren, Man,Hu, Qianqian,Gu, Youfang,Li, Shenghe,Li, Kui.

[6]Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Zhang, WY,Moreau, E,Yang, BZ,Li, ZQ,Hope, JC,Howard, CJ,Huang, WY,Chauvin, A. 2006

[7]Antiviral effect of recombinant equine interferon-gamma on several equine viruses. Sentsui, Hiroshi,Wu, Donglai,Murakami, Kenji,Kondo, Takashi,Matsumura, Tomio. 2010

[8]Effects of different doses of free alpha-linolenic acid infused to the duodenum on the immune function of lactating dairy cows. Sun, Peng,Wang, Jiaqi,Yang, Guang,Liu, Qingsheng.

[9]Course of infection by Babesia sp BQ1 (Lintan) and B-divergens in sheep depends on the production of IFN gamma and IL10. Moreau, E.,Guan, G.,Yin, H.,Luo, J.. 2010

[10]Expression of biologically active recombinant equine interferon-gamma in Escherichia coli. Bai, Yu,Tong, Tiegang,Liu, Guangliang,Chen, Weiye,Zhang, Weijun,Wang, Qun,Yang, Tao,Bu, Zhigao,Wu, Donglai,Bai, Yu. 2010

[11]Comparative transcriptome analysis of MDBK cells reveals that BoIFN-γ augmented host immune responses to bovine herpesvirus 1 infection. Bo Jiang,Jing Wang,Wenxiao Liu,Jing Cheng,Jian Xu,Mengyao Cao,Yongqing Li. 2022

[12]Effects of different dietary protein levels and DL-methionine supplementation on hair growth and pelt quality in mink (Neovision vision). Zhang, H. H.,Jiang, Q. K.,Sun, W. L.,Xu, C.,Cong, B.,Yang, F. H.,Li, G. Y.. 2013

[13]Cloning, expression and antiviral activity of mink alpha-interferons. Zhang, Hai-ling,Zhao, Jian-jun,Chai, Xiu-li,Zhang, Lei,Bai, Xue,Hu, Bo,Liu, Hao,Yan, Xi-jun,Zhang, Dong-liang,Ye, Ming,Wu, Wei. 2015

[14]Detection and Characterization of Avastrovirus Associated with Diarrhea Isolated from Minks in China. Sun, Na,Yang, Yong,Shao, Xi-Qun,Zhang, Shu-Qin,Wang, Feng-Xue,Tan, Bin,Cheng, Shi-Peng,Wen, Yong-Jun,Wang, Gui-Sheng,Tian, Fu-Lin.

[15]Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Zhao, Jian-Jun,Yan, Xi-Jun,Chai, Xiu-Li,Luo, Guo-Liang,Zhang, Hai-Ling,Gao, Han,Bai, Xue,Zhang, Lei,Chen, Tao,Xu, Lei,Zhao, Chun-Fei,Wang, Feng-Xue,Shao, Xi-Qun,Wu, Wei,Cheng, Shi-Peng,Martella, Vito,Liu, Ying-Xue.

[16]Effects of Different Dietary Manganese Levels on Growth Performance and N Balance of Growing Mink (Neovision vision). Zhang, H. H.,Zhou, N.,Zhang, T. T.,Bao, K.,Xu, C.,Song, X. C.,Zhou, N..

[17]Copper bioavailability, blood parameters, and nutrient balance in mink. Wu, X. Z.,Guo, J. G.,Liu, Z.,Gao, X. H.,Wu, X. Z.,Zhang, T. T.,Yang, F. H.,Yang, F. H..

[18]Detection of Mink (Mustela vison) DNA in Meat Products using Polymerase Chitin Reaction PCR Assay. Sun, Weili,Li, Guangyu,Liu, Hanlu,Zhong, Wei,Zhang, Haihua,Bao, Kun,Xu, Chao,Yang, Yahan,Wang, Zhuo,Sun, Weili,Li, Guangyu,Liu, Hanlu,Zhong, Wei,Zhang, Haihua,Bao, Kun,Xu, Chao,Wang, Zhuo,Yang, Yahan. 2012

[19]Effects of dietary copper on nutrient digestibility, tissular copper deposition and fur quality of growing-furring mink (Mustela vison). Wu, Xuezhuang,Liu, Zhi,Gao, Xiuhua,Wu, Xuezhuang,Zhang, Tietao,Yang, Fuhe,Wu, Xuezhuang,Zhang, Tietao,Yang, Ying,Yang, Fuhe.

[20]Influence of Dietary Zinc and Copper on Apparent Mineral Retention and Serum Biochemical Indicators in Young Male Mink (Mustela vison). Wu, Xuezhuang,Liu, Zhi,Guo, Jungang,Wan, Chunmeng,Cui, Hu,Gao, Xiuhua,Wu, Xuezhuang,Zhang, Tietao,Yang, Fuhe.

作者其他论文 更多>>