数字农科院2.0

OBP14 (Odorant-binding protein) sensing in Adelphocoris lineolatus based on peptide nucleic acid and graphene oxide

文献类型： 外文期刊

作者： Tian Wenhua;Zhang Tao;Gu Shaohua;Guo Yuyuan;Gao Xiwu;Zhang Yongjun

作者机构：

关键词： Adelphocoris lineolatus; AlinOBP14; Graphene oxide (GO); In vitro; In vivo; Juvenile hor-mone; MRNA biosensor; Peptide nucleic acid (PNA); Plus-C OBPs; Terpenoids

期刊名称： Insects

ISSN： 2075-4450

年卷期： 2021 年 12 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in A. lineolatus, the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on AlinOBP14, a Plus-C OBP from A. lineolatus, and we developed a PNA-GO-based mRNA biosensor to detect the expression of AlinOBP14. The results demonstrated that AlinOBP14 plays dual roles in A. lineolatus. The AlinOBP14 is expressed beneath the epidermis of the vertex and gena in heads of A. lineolatus, and it functions as a carrier for three terpenoids, while AlinOBP14 is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHIII. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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