数字农科院2.0

Deletion of the 3′ End of the Introduced cry1Ac Gene Retains the Insecticidal Activity in Transgenic Cotton

文献类型： 外文期刊

作者： Chunmeng Huang;Yongjun Zhang;Huilin Yu;Chenhui Hou;Haoyuan Guan;Xiuping Chen;Jiajian Xie

作者机构：

关键词： ELISA;insect-resistant;LC50;MON531;R7569;RACE;RT-PCR;transgenic cotton

期刊名称： Agronomy

ISSN： 2073-4395

年卷期： 2024 年 14 卷 7 期

页码：

收录情况： SCIE（2024版）

摘要： The structure and expression of exogenous genes in transgenic crops are critical for the target traits. R7569 has the same exogenous insertion structure as the transgenic insect-resistant cotton MON531 but with a deletion in the 3′ end of the cry1Ac gene and the terminator region. Thus, in the present study, transcription, expression, and insecticidal activity assays were conducted to determine the function of the truncated cry1Ac gene. R7569 has a truncated cry1Ac gene with a length of 2554 bp encoding 881 amino acids, and the transcription termination site was mainly concentrated downstream of the truncated position and extended 160–270 bp from the truncated position using rapid amplification of cDNA ends (RACE). The transcript levels of the cry1Ac gene in R7569 were significantly higher than those of MON531 implants, except for during the boll stage. The content of the Cry1Ac protein in R7569 was higher than that of MON531 in the cotton leaf in all three periods. The corrected mortality rates of R7569 and MON531 against bollworms were 93.09% and 88.83%, respectively. The LC50 value of R7569 was 0.732 ng/g (dw), indicating a high level of resistance to bollworm. In this study, for the first time, we found a partial deletion of the target gene in commercially applied transgenic crops, and the partial deletion of the 3′ end of the cry1Ac gene retained a better transcription, expression level, and insecticidal activity, which can provide a specific case for the safety evaluation of transgenic crops.

分类号：

  • 相关文献

[1]Cloning and characterization of gene encoding a Mn-containing superoxide dismutase in Eutrema halophilum. Xu, X. J.,Zhou, Y. J.,Bu, H. H.,Feng, J. C.,Ren, D. T.,Wang, G. Y.. 2014

[2]Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome in Jiangxi province, China. Guo, Aijiang,Wu, Guohua,Gong, Wei,Luo, Xuenong,Zheng, Haixue,Jia, Huanjie,Cai, Xuepeng. 2012

[3]陆地棉耐盐相关基因(GhVP)的克隆及分析. 叶武威,赵云雷,王俊娟,樊保香,王德龙. 2010

[4]镰形扇头蜱IgG结合蛋白基因的克隆与表达分析. 万修红,周勇志,向飞宇,张文杰,周金林. 2007

[5]草地螟触角普通气味结合蛋白基因的克隆及序列分析. 尹姣,刘怀,王进军,李克斌,韦永贵,曹雅忠. 2008

[6]差异显示和RACE技术的发展与应用. 孔凡晶,马有志,陈孝,辛志勇. 2003

[7]紫花苜蓿LEA第3组基因(MsLEA3-1)的克隆与分析. 金后聪,杨青川,孙彦,康俊梅,晁跃辉,金洪,龙瑞才,李燕. 2010

[8]牛FABGL基因cDNA的克隆与序列分析. 马云,许尚忠,高雪,张英汉,辛亚平,高树新,任红艳. 2006

[9]济南地区倒瓤西瓜CGMMV的检测. 刘颖,彭斌,杨晓红,李向东,古勤生. 2012

[10]利用ELISA和RT-PCR法检测甜菜丛根病毒(BNYVV). 李泽,王洪超,吴玉梅,张福顺. 2008

[11]羊痘病毒诊断学研究进展. 王曼,卢昌,赵志荀,吴国华,颜新敏,朱海霞,李健,张强. 2014

[12]我国葡萄主栽区卷叶病相关病毒种类的检测分析. 裴光前,董雅凤,张尊平,范旭东,任芳. 2011

[13]红将军苹果的脱毒与检测技术研究. 苏佳明,段小娜,于强,徐维华,姜中武,董雅凤,陈学森. 2009

[14]毛皮动物新冠病毒监测简报. 曹海旭,胡博,张海玲,李双双,李虹晔,赵传芳,廉士珍,刘昊,鲁荣光,白雪. 2020

[15]跨国公司生物技术知识产权保护策略分析——以棉花转化体MON531为例. 宋敏,刘娜,任欣欣,卢垚. 2014

[16]不同地区苹果绵蚜对三种杀虫剂的抗性监测. 张昌振,郭磊,陈麒兆,杜凌君,周昊,万方浩,周洪旭. 2019

[17]2015—2021年亚洲玉米螟对Cry1Ie杀虫蛋白的敏感性监测. 王月琴,静大鹏,刘凯强,王振营,何康来. 2024

[18]siRNA induced DNA methylation of the cry1Ac gene due to its inverted duplication in genetically modified cotton MON531. 黄春蒙, 陈秀萍, 于惠林, 侯晨辉, 关浩源, 谢家建. 2025

[19]Acute toxicity of five pesticides to Apis mellifera larvae reared in vitro. Dai, Pingli,Dai, Pingli,Jack, Cameron J.,Mortensen, Ashley N.,Ellis, James D..

[20]The in vitro efficacy of deltamethrin and alpha-cypermethrin against "engorged female Haemaphysalis qinghaiensis ticks (Acari: Ixodidae). Ma, Miling,Guan, Guiquan,Liu, Qing,Dang, Zhisheng,Liu, Aihong,Ren, Qiaoyun,Liu, Zhijie,Li, Youquan,Chen, Ze,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun. 2013

作者其他论文 更多>>