数字农科院2.0

ICECleSHZ29: Novel Integrative and Conjugative Element (ICE)-Carrying Tigecycline Resistance Gene tet(X6) in Chryseobacterium lecithinasegens

文献类型： 外文期刊

作者： Chen, Xi;Zhang, Yifei;Jiang, Chunling;Lin, Yafang;Yao, Xiaohui;Nie, Wansen;Li, Lin;Wei, Jianchao;Shao, Donghua;Liu, Ke;Li, Zongjie;Qiu, Yafeng;Ma, Zhiyong;Li, Beibei;Xia, Lining

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关键词： integrative and conjugative element;antibiotic resistance;tet(X6);Chryseobacterium;swine

期刊名称： ANTIBIOTICS-BASEL

ISSN： 2079-6382

年卷期： 2025 年 14 卷 10 期

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收录情况： SCIE（2025版）

摘要： Background/Objectives: The global dissemination of tet(X) variants critically threatens tigecycline efficacy as a last-resort antibiotic. The aim of this study was to characterize a tet(X6)-carrying integrative and conjugative element (ICE) in a multidrug-resistant Chryseobacterium lecithinasegens strain, SHZ29, isolated from Shanghai, China. Methods: Minimum inhibitory concentrations (MICs) were determined by broth microdilution for SHZ29. Whole genomic sequencing and bioinformatic analysis were performed to depict the structure of the novel tet(X6)-carrying ICE. Inverse PCR and conjugation experiments were conducted to investigate the transfer ability of the ICE. Results: We depicted a novel tet(X6)-carrying ICE, named ICECleSHZ29, which is 74,906 bp in size and inserted into the 3 ' end of tRNA-Met-CAT gene of the C. lecithinasegens strain SHZ29, with 17 bp direct repeats (5 '-tcccgtcttcgctacaa-3 '). This ICE possesses a 38 kb conserved backbone and four variable regions (VR1-4), with VR3 aggregating multiple resistance genes, including tet(X6), tet(X2), erm(F), ere(D), floR, catB, sul2, ant(6)-I and blaOXA-1327. NCBI database searching identified 13 additional ICEs sharing a similar backbone to ICECleSHZ29. These ICECleSHZ29-like ICEs could be classified into two types based on their distinct insertion sites: Type I, inserted at the tRNA-Met-CAT gene; and Type II, inserted at the tRNA-Glu-TTC gene. Phylogenetic analysis indicated that differences in integrases may result in differences in the insertion site among these ICEs. A circular intermediate form of ICECleSHZ29 was detected by inverse PCR. However, the conjugation experiments using Escherichia coli EC600 as recipients failed. Conclusions: To our knowledge, this study provides the first report of tet(X6) in C. lecithinasegens and characterizes its carrier, a novel ICE: ICECleSHZ29.

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