数字农科院2.0

A Novel Laboratory Protocol for Pollen Viability Assessment to Inform Biosafety Evaluation of Transgenic Rice (Oryza sativa L.)

文献类型： 外文期刊

作者： Yuxiao Chen;Caiyue Liu;Xiaochun Zhang;Yufeng Dong;Jiangtao Yang;Dongmei Wang;Zhixing Wang;Xujing Wang

作者机构：

关键词： biosafety policy;pollen viability;rice (Oryza sativa L.)

期刊名称： Agriculture (Switzerland)

ISSN：

年卷期： 2025 年 15 卷 23 期

页码：

收录情况： SCIE（2025版）

摘要： Rice (Oryza sativa L.) is a vital staple crop, and the environmental risk assessment of transgenic varieties is crucial for formulating biosafety policies. Rice pollen grains are spherical, with an average diameter of 40.03 ± 2.75 μm. This study established a standardized protocol for in vitro pollen germination by first optimizing key culture conditions. A single-factor experimental design identified the optimal medium composition as 150 g/L sucrose, 40 mg/L boric acid, 20 mg/L calcium chloride, 10 mg/L monopotassium phosphate, and 10 mg/L magnesium sulfate. The ideal germination temperature was determined to be 31 ± 1 °C, with no germination observed below 16 °C or above 40 °C. Pollen germination rates declined significantly within 5 min post-isolation and ceased completely after 30 min. Building on this optimized protocol, a standardized evaluation method was developed, defining key assessment conditions at temperatures of 25/31/37 °C and post-isolation times of 0/5/15 min. Under these defined conditions, the pollen viability of glyphosate-resistant transgenic rice G2-6 was compared to its non-transgenic recipient ZH11. No significant differences were found at any tested time–temperature combination (p > 0.05). This work establishes a practical and reproducible standard for transgenic rice pollen assessment, offering a scientific basis for evidence-based biosafety regulation and policy-making.

分类号：

  • 相关文献

[1]Genetic Dissection for Leaf Correlative Traits of Rice (Oryza sativa L.)Under Drought Stress. Guo Longbiao,Qian Qian,Zeng Dali,Dong Guojun,Teng Sheng,Zhu Lihuang. 2004

[2]GLUCAN SYNTHASE-LIKE 5 (GSL5) Plays an Essential Role in Male Fertility by Regulating Callose Metabolism During Microsporogenesis in Rice. Shi, Xiao,Sun, Xuehui,Zhang, Zhiguo,Feng, Dan,Zhang, Qian,Han, Lida,Wu, Jinxia,Lu, Tiegang.

[3]Pollen Semi-Sterility1 Encodes a Kinesin-1-Like Protein Important for Male Meiosis, Anther Dehiscence, and Fertility in Rice. Zhou, Shirong,Wang, Yang,Li, Wanchang,Zhao, Zhigang,Ren, Yulong,Wang, Yong,Jiang, Ling,Liu, Linglong,Wan, Jianmin,Zhou, Shirong,Gu, Suhai,Lin, Qibing,Wang, Dan,Su, Ning,Zhang, Xin,Cheng, Zhijun,Lei, Cailin,Wang, Jiulin,Guo, Xiuping,Wu, Fuqing,Wang, Haiyang,Wan, Jianmin,Ikehashi, Hiroshi.

[4]Pollen viability and stigma receptivity in Lilium during anthesis. He, Guoren,Ming, Jun,Liu, Chun,Yuan, Suxia,He, Guoren,Hu, Fengrong.

[5]Shade Delayed Flowering Phenology and Decreased Reproductive Growth of Medicago sativa L.. Qin, Fengfei,Shen, Yixin,Li, Zhihua,Qu, Hui,Feng, Jinxia,Kong, Lingna,Teri, Gele,Luan, Haoming,Cao, Zhiling. 2022

[6]Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice. Wang Tao,Lou Lijuan,Li Zeyu,Shang Lianguang,Wang Quan. 2021

[7]Pollen grain number and viability contribute to variation in effective ovule number among oilseed rape genotypes. Xiao Guo,Bao Luo Ma,Neil B. McLaughlin,Yajun Gao,Xiaoming Wu,Biyun Chen. 2022

[8]A Novel Laboratory Protocol for Pollen Viability Assessment to Inform Biosafety Evaluation of Transgenic Rice. 陈宇枭,,刘彩月,,张晓春,,董玉凤,,王冬梅,,杨江涛,,王志兴,,王旭静. 2025

[9]Development and Application of an In Vitro Pollen Viability Assay for Comparative Safety Assessment of Transgenic Alfalfa (Medicago sativa L.). Chen, Yuxiao,Zhang, Xiaochun,Yang, Jiangtao,Guo, Diandian,Wang, Xujing,Wang, Zhixing. 2025

[10]Optimization of grape artificial hybrid pollination technology process and the effect of different male parents on the fruit setting rate. Zhou, Ruijin,Gai, Ruxin,Liao, Wanying,Wu, Qiujie,Cheng, Jiahui,Li, Yongmu,Fan, Xiucai,Li, Guirong. 2025

[11]Effect Of Various Crop Rotations O.n Rice Yield And N itrogen Use Efficiency In Paddy-Upland Systems In Southeastern China. Zhang, Xiufu,Yin, Min,Liu, Shaowen,Xu, Chunmei,Wang, Danying,Yan, Jinxiang,Chu, Guang,Chen, Song,Chen, Song,Chen, Liping,Zheng, Xi. 2018

[12]Suppression Of Ros Generation Mediated B.y Higher Insp(3) Level I s Critical For The Delay Of Seed Germination In Lpa Rice. Zakari, Shamsu Ado,Ye, Yu,Cheng, Fangmin,Zhao, Qian,Cheng, Fangmin,Pan, Gang,Du, Xiaoxia,Su, Da,Zhao, Qian,Zhou, Lujian. 2018

[13]Identification Of Qtls Associated With H.eat Tolerance At The H eading And Flowering Stage In Rice (Oryza Sativa L.). Liu, Jin,Wu, Jin-wen,Song, Youchun,Shin, Dongjin,Yu, Li-qin,Park, Dong-Soo,Li, Xia,Jo, Su-min,Li, Mao-mao,Ma, Xiao-ding,Li, Mao-mao,Li, Hui,Han, Long-zhi. 2018

[14]Cyclin-Dependent Kinase Inhibitors Krp1 And Krp2 Are Involved In Grain Filling And Seed Germination In Rice (Oryza Sativa L.). Tong, Xiaohong,Zhao, Juan,Wang, Huimei,Wang, Shuang,Ajadi, Abolore Adijat,Li, Shufan,Ajadi, Abolore Adijat,Tang, Liqun,Liu, Xixi,Wang, Shuang,Shu, Yazhou,Liu, Wanning,Tajo, Sani Muhammad,Li, Zhiyong,Wang, Yifeng,Zhang, Jian. 2020

[15]Sulfur Supply Reduces Cadmium Uptake A.nd Translocation In Rice G rains (Oryza Sativa L.) By Enhancing Iron Plaque Formation, Cadmium Chelation And Vacuolar Sequestration. Lin, Xiao-Yan,Chen, Ming-Xue,Zhu, Zhi-Wei,Qin, Mei-Ling,Cao, Zhen-Zhen. 2018

[16]Different Phosphorus Supplies Altered The A.ccumulations And Quantitative Distributions O f Phytic Acid, Zinc, And Iron In Rice (Oryza Sativa L.) Grains. Zhao, Qian,Zhou, Lujian,Cheng, Fangmin,Su, Da,Pan, Gang,Cheng, Fangmin,Su, Da. 2018

[17]Effects of Mn-Cd antagonistic interaction on Cd accumulation and major agronomic traits in rice genotypes by different Mn forms. Huang, Qi-na,Yang, Yong-jie,Liang, Yan,Shao, Guo-sheng,An, Hua.

[18]Polyphenols in whole rice grain: Genetic diversity and health benefits. Shao, Yafang,Shao, Yafang,Bao, Jinsong.

[19]Validation and dissection of quantitative trait loci for leaf traits in interval RM4923-RM402 on the short arm of rice chromosome 6. Shen, Bo,Yu, Wei-Dong,Du, Jing-Hong,Fan, Ye-Yang,Wu, Ji-Rong,Zhuang, Jie-Yun,Shen, Bo,Yu, Wei-Dong,Du, Jing-Hong,Fan, Ye-Yang,Wu, Ji-Rong,Zhuang, Jie-Yun,Shen, Bo,Yu, Wei-Dong,Du, Jing-Hong.

[20]Comprehensive expression of various genes involved in storage protein synthesis in filling rice grain as affected by high temperature. Cao, Zhenzhen,Cao, Zhenzhen,Zhao, Qian,Pan, Gang,Wei, Kesu,Zhou, Lujian,Cheng, Fangmin.

作者其他论文 更多>>