数字农科院2.0

Disinfection mechanism of chlorine-resistant bacteria by micro-nano bubbles in drinking water: A case study of Bacillus cereus

文献类型： 外文期刊

作者： Yang Ci;Wang Tianzhi;Luo Peiyuan;Fiallos Manuel;Guo Suxia;Zhao Yujie

作者机构：

关键词： Bacillus cereus;Drinking water biosafety;Micro-nano bubbles;Sterilization mechanism

期刊名称： Chemical Engineering Journal

ISSN： 1385-8947

年卷期： 2025 年 515 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Aiming to address the challenge of eliminating chlorine-resistant pathogens, particularly Bacillus cereus (B. cereus), in drinking water treatment, this study developed a novel disinfection technology based on micro-nano bubbles (MNBs). A systematic comparison of the bactericidal effects of air-, O3-, and N2- MNBs elucidated the unique mechanism by which MNBs inactivated bacteria through physico-chemical synergies. Experimental results demonstrated that MNBs could be generated using various gas sources in water, with bubble concentrations reaching over 10⁶ bubbles/mL. The hydroxyl radicals ([rad]OH) produced during the collapse of MNBs exhibited zero-order kinetic behavior, achieving radical concentrations up to 335 μmol/L. Upon entering the water, MNBs first dispersed bacterial populations, disrupting microbial cooperation. Subsequently, the [rad]OH generated during bubble collapse directly and indirectly eliminated bacteria by degrading nutrients in water and attacking bacterial cells. After MNB treatment, total organic carbon (TOC) content decreased by 8.89 %–16.23 %, and bacterial inactivation efficiency reached 85.35 %–99.91 %. Bacterial cell membranes were observed to rupture, leading to intracellular leakage. Simultaneously, a considerable influx of water into the cell caused cellular expansion and stretching, resulting in an increase in bacterial volumn by 70.37 %–93.00 %. Oxidative damage induced by [rad]OH resulted in DNA denaturation, protein secondary structure disruption, and eventual bacterial death. This research not only provides a new theoretical foundation for the application of MNB technology in water treatment but also offers an effective technical pathway for addressing chlorine-resistant pathogens, showcasing broad application prospects and practical significance.

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