数字农科院2.0

Molecular-level degradation of antibiotic resistance genes in electrochemical oxidation: Targeting promoter regions

文献类型： 外文期刊

作者： Qiu, Longmei;Zhang, Jing;Liang, Chengzhen;Li, Hongna

作者机构：

关键词： Antibiotic resistance genes;Electrochemical Oxidation;Molecular-level degradation;Promoter region;Genome mapping

期刊名称： JOURNAL OF HAZARDOUS MATERIALS

ISSN： 0304-3894

年卷期： 2025 年 502 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The escalating global spread of antibiotic resistance poses a critical threat to public health. Electrochemical oxidation (EO) has already shown excellent efficacy in inactivation of antibiotic-resistant bacteria and removal of antibiotic resistance genes (ARGs). However, the evolution pathway of ARGs in molecular scale is still unclear, which limits the accurate risk assessment of antibiotic resistance. In this work, degradation processes of three typical ARGs were investigated (sul1, tet(M), tet(O)) in EO treatment with titanium electrodes. It was observed that the abundance of all three ARGs was effectively reduced, with tet(M) exhibiting the highest susceptibility (0.0086 s-1),followed by tet(O) (0.0068 s-1) and sul1 (0.0035 s-1). High-throughput sequencing revealed a dynamic degradation trajectory characterized by progressive ARG fragmentation and accumulation of Apurinic/apyrimidinic (AP) sites. ARG fragments spanning 51-148 bp steadily decreased initially, and short fragments concentrated in 48-50 bp dominated in later-stage. Motif analysis revealed specific degradation-prone sequences, suggesting that structural and functional differences among ARGs significantly influence their susceptibility to electrochemical degradation. In addition, genomic mapping of these motifs demonstrated that EO preferentially targeted promoter regions of ARGs (91.03 %-92.19 %), leading to the disruption of genes involved in transcriptional regulation, amino acid metabolism, and bacterial stress responses. Especially, tet(M) exhibited pronounced time-dependent shifts from ribosomal synthesis to amino acid metabolism, while degradation of tet(O) remained temporally stable. This study elucidates the degradation pathway of typical ARGs in EO treatment, providing insights into the molecular mechanisms and genomic impacts, and highlighting a knowledge-driven approach to mitigate the transmission risks of antibiotic resistance.

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