数字农科院2.0

Functional characterization of pheromone receptor candidates in codling moth Cydia pomonella

文献类型： 外文期刊

作者： Ke Tian#; Wei Liu#; Likai Feng; Tianyu Huang; Guirong Wang*; Kejian Lin*

期刊名称： Insect Science

ISSN： 1672-9609

年卷期： 2022 年

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： Sex pheromones serve a critical role in Lepidopterans finding mates. Male moths perceive and react to sex pheromones emitted by conspecific females through a delicate pheromone communication system. Pheromone receptors (PRs) are the key sen- sory elements at the beginning of that process. The codling moth (Cydia pomnonella) is an important pome fruit pest globally and a serious invasive species in China. Pheromone- based techniques have been used successfully in monitoring and controlling this species. We conducted ribonucleic acid sequencing analysis of the codling moth antennal transcrip- tome and identified 66 odorant receptors (ORs) in a population from Xinjiang province, China, of which 14 were PRs, including two novel PRs (CpomOR2e and CpomOR73). Four PRs that contain full-length open reading frames (CpomOR1, OR2a, OR5, OR7) and four PRs with ligands that have not been reported previously (CpomOR1, OR2a, OR5, OR7) were selected to deorphanize in the heterologous Xenopus oocyte expres- sion system. Specifically, we found that CpomOR2a and CpomOR5 responded to (E, E)-8, 10-dodecadien-1-yl acetate (codlemone acetate). Furthermore, CpomOR5 (EC 50 = 1.379 × 10−8 mol/L) was much more sensitive to codlemone acetate than CpomOR2a (EC50 = 1.663 × 10−6 mol/L). Since codlemone acetate is an important component of C. pomonella sex pheromone, our results improve the current understanding of pheromone communication in codling moths and will be helpful for the development of pest manage- ment strategies.

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