数字农科院2.0

Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila

文献类型： 外文期刊

作者： Jinyang Li;Yidi Wang;Kun Yang;Xiaolu Wang;Yuan Wang;Honglian Zhang;Huoqing Huang;Xiaoyun Su;Bin Yao;Huiying Luo;Xing Qin

作者机构：

关键词： 5’UTR;Myceliophthora thermophila;Promoter;Protein expression;Signal peptide

期刊名称： Microbial Cell Factories

ISSN： 1475-2859

年卷期： 2023 年 22 卷 1 期

页码：

收录情况： SCIE（2023版）

摘要： Background: Thermophilic fungus Myceliophthora thermophila has been widely used in industrial applications due to its ability to produce various enzymes. However, the lack of an efficient protein expression system has limited its biotechnological applications. Results: In this study, using a laccase gene reporting system, we developed an efficient protein expression system in M. thermophila through the selection of strong constitutive promoters, 5’UTRs and signal peptides. The expression of the laccase was confirmed by enzyme activity assays. The results showed that the Mtpdc promoter (Ppdc) was able to drive high-level expression of the target protein in M. thermophila. Manipulation of the 5’UTR also has significant effects on protein expression and secretion. The best 5’UTR (NCA-7d) was identified. The transformant containing the laccase gene under the Mtpdc promoter, NCA-7d 5’UTR and its own signal peptide with the highest laccase activity (1708 U/L) was obtained. In addition, the expression system was stable and could be used for the production of various proteins, including homologous proteins like MtCbh-1, MtGh5-1, MtLPMO9B, and MtEpl1, as well as a glucoamylase from Trichoderma reesei. Conclusions: An efficient protein expression system was established in M. thermophila for the production of various proteins. This study provides a valuable tool for protein production in M. thermophila and expands its potential for biotechnological applications.

分类号：

  • 相关文献

[1]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[2]拟南芥VTC1的5’UTR内含子功能分析. 李生辉,王凤茹,黄荣峰,董金皋,王娟. 2016

[3]Discovering Functional Diversity of Lytic Polysaccharide Monooxygenases from the Thermophilic Fungus Myceliophthora Thermophila and Their Application in Lignocellulosic Biomass Degradation. Qin, Xing,Zou, Jiahuan,Yang, Kun,Li, Jinyang,Wang, Xiaolu,Tu, Tao,Zhang, Jie,Su, Xiaoyun,Yao, Bin,Huang, Huoqing,Luo, Huiying. 2023

[4]Boosting the cellulolytic oxidative and hydrolytic enzyme systems in Myceliophthora thermophila for the efficient enzymatic saccharification of lignocellulosic biomass. Bai, Rui,Yang, Kun,Wang, Yaru,Wang, Yuan,Wang, Xiaolu,Tu, Tao,Zhang, Jie,Su, Xiaoyun,Huang, Huoqing,Yao, Bin,Luo, Huiying,Qin, Xing. 2025

[5]Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR. Zhang, Yi,Tian, Baozhong,Guo, Tingqing,Wang, Jianyang,Wei, Zhenguo,Lu, Changde,Zhang, Yi,Xia, Huanzhang,Tian, Baozhong,Wei, Zhenguo,Wang, Shengpeng.

[6]Genetic and biochemical characterization of a protease-resistant mesophilic beta-mannanase from Streptomyces sp S27. Shi, Pengjun,Yuan, Tiezheng,Zhao, Junqi,Huang, Huoqing,Luo, Huiying,Meng, Kun,Wang, Yaru,Yao, Bin.

[7]In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector. Wang, SP,Guo, TQ,Guo, XY,Huang, JT,Lu, CD.

[8]Bioinformatics Analysis and Functional Characterization of the CFEM Proteins of Metarhizium anisopliae. Ni Cai,Rong Liu,Duozi Yan,Neng Zhang,Kaihui Zhu,Daogang Zhang,Xiangqun Nong,Xiongbing Tu,Zehua Zhang,Guangjun Wang. 2022

[9]Exploiting Systematic Engineering of the Expression Cassette as a Powerful Tool to Enhance Heterologous Gene Expression in Trichoderma reesei. Ji, Wangli,Xu, Li,Sun, Xianhua,Xu, Xinxin,Zhang, Honglian,Luo, Huiying,Yao, Bin,Zhang, Wei,Su, Xiaoyun,Huang, Huoqing. 2024

[10]Integrative approaches unravelling tea drought alleviation mechanisms primed by carbonyl volatiles and signal peptide. Abdelkader Bassiony,Shujuan Sun,Kangni Yan,Qunhua Peng,Mengxue Zhou,Jianyu Fu,Susanne Baldermann,Jiang Shi,Haipeng Lv,Zhi Lin. 2025

[11]Expression of proteins in superior and inferior spikelets of rice during grain filling under different irrigation regimes. Chen, Tingting,Xu, Genwen,Wang, Zhiqin,Zhang, Hao,Yang, Jianchang,Chen, Tingting,Zhang, Jianhua,Zhang, Jianhua.

[12]Thioredoxin reductase from Toxoplasma gondii: an essential virulence effector with antioxidant function. Xue, Junxin,Jiang, Wei,Chen, Yongjun,Wang, Minyan,Wang, Quan,Xue, Junxin,Gong, Fengju,Zeng, Peng,Xia, Can,Huang, Kehe,Xue, Junxin.

[13]Proteomic analysis of PEG-simulated drought stress-responsive proteins of rice leaves using a pyramiding rice line at the seedling stage. Xiong, Jian-Hua,Li, Yang-Sheng,Xiong, Jian-Hua,Fu, Bin-Ying,Xu, Hua-Xue.

[14]Cloning and expression analysis of a peptidoglycan recognition protein in silkworm related to virus infection. Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[15]Molecular cloning of a full-length cDNA for ECBP21 from Angelica dahurica. Mao, GH,Tang, WQ,Guo, Y,Ding, CB,Zhou, RG,Sun, DY. 2002

[16]FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE). Wang, Gui-Rong,Zhang, Xue-Ying,Guo, Yu-Yuan,Zhang, Yong-Jun,Wang, Wei-Xuan,Zhang, Ziding,Zhou, Jing-Jiang. 2011

[17]Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Chu, Xiao-Yu,Wu, Ning-Feng,Deng, Min-Jie,Tian, Jian,Yao, Bin,Fan, Yun-Liu.

[18]Regulation of cry Gene Expression in Bacillus thuringiensis. Peng, Qi,Song, Fuping,Deng, Chao,Lereclus, Didier,Lereclus, Didier. 2014

[19]Characterization of M-class genome segments of muscovy duck reovirus S14. Zhang, Yun,Guo, Dongchun,Geng, Hongwei,Liu, Ming,Hu, Qilin,Wang, Junwei,Tong, Guangzhi,Kong, Xiangang,Liu, Nihong,Liu, Chunguo. 2007

[20]Binding Characterization of Recombinant Odorant-binding Proteins from the Parasitic Wasp, Microplitis mediator (Hymenoptera: Braconidae). Zhang, Shuai.

作者其他论文 更多>>