数字农科院2.0

A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)

文献类型： 外文期刊

作者： Wang, L. H.;Gu, X. H.;Hua, M. Y.;Mao, S. L.;Zhang, Z. H.;Zhang, B. X.;Gu, X. H.;Hua, M. Y.;Yun, X. F.;Peng, D. L.

作者机构：

关键词： Pepper;Capsicum annuum L.;Disease resistance;Root-knot nematode;Meloidogyne spp.;SCAR;N gene;Marker assisted selection

期刊名称： SCIENTIA HORTICULTURAE

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收录情况： SCI

摘要： The root-knot nematodes (Meloidogyne spp.) are important nematode pests and cause serious diseases in pepper in the world. No molecular markers linked to the nematodes resistance N gene have been reported. In this paper, 'Carolina Wonder' (Capsicum annuum L.), a sweet pepper line resistant to root-knot nematode with N gene, '20080-5-29' (C. annuum L.), an inbred line susceptible to root-knot nematode with good horticultural characteristics, and their F progeny with 320 individuals were used as materials. Evaluation of resistance and susceptibility of parental lines, F and F progeny inoculated with root-knot nematodes (Meloidogyne incognita) were carried out. 'Bulked segregant analysis' method was used to search for polymorphic markers from 512 pairs of AFLP primers. Based on the assessment of resistance and susceptibility and polymorphism of the AFLP marker in F population, the genetic linkage distance between the AFLP marker and the N gene was estimated. One AFLP marker E39/M41-339 was obtained and transferred to a SCAR marker amplifying a 315bp DNA fragment linked to the N resistant allele and a 331bp fragment linked to the N susceptible allele. The distance between the molecular marker and the nematodes resistance N gene is 6.3cM. This research delivered a valuable tool for the marker assisted selection of nematodes resistance in pepper.

分类号： S6

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