数字农科院2.0

LncRNA ENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR-146b/AGPAT2 pathway

文献类型： 外文期刊

作者： Xing, Wenhao;Li, Shijie

作者机构：

关键词： AGPAT2;chicken;fat metabolism;hepatocyte;lncRNA;microRNA

期刊名称： ANIMAL GENETICS

ISSN： 0268-9146

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： The liver contributes to lipid metabolism as the hub of fat synthesis. Long non-coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes' lipid metabolism. Thirty-two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR-146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)-146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high-fat diet modulated the expression of lncRNA 21 686 and miR-146b (p-value < 0.001). Moreover, there was a significant increase in 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat-related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p-value < 0.05). Overexpression of miR-146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR-146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR-146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.

分类号：

  • 相关文献

[1]哺乳动物毛囊发育及调控研究进展. 郭雪峰,包鹏甲,常永芳,张永峰,李忠邦,雷蕾,阎萍,潘和平. 2019

[2]RNA-Seq Reveals Pathways Responsible for Meat Quality Characteristic Differences between Two Yunnan Indigenous Chicken Breeds and Commercial Broilers. Yong Liu,Xia Zhang,Kun Wang,Qihua Li,Shixiong Yan,Hongmei Shi,Lixian Liu,Shuangmin Liang,Min Yang,Zhengchang Su,Changrong Ge,Junjing Jia,Zhiqiang Xu,Tengfei Dou. 2024

[3]Plant non-coding RNAs: The new frontier for the regulation of plant development and adaptation to stress. Wenxue Cao,Limei Yang,Mu Zhuang,Honghao Lv,Yong Wang,Yangyong Zhang,Jialei Ji. 2024

[4]Discovery of novel long non-coding RNAs induced by subgroup J avian leukosis virus infection in chicken. Qiu, Lingling,Li, Zhiteng,Chang, Guobin,Bi, Yulin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[5]Genetic Basis of Sexual Maturation Heterosis: Insights From Ovary lncRNA and mRNA Repertoire in Chicken. Wang Y.,Yuan J.,Sun Y.,Li Y.,Wang P.,Shi L.,Ni A.,Zong Y.,Zhao J.,Bian S.,Ma H.,Chen J.. 2022

[6]Dual RNA-Seq of H5N1 Avian Influenza Virus and Host Cell Transcriptomes Reveals Novel Insights Into Host-Pathogen Cross Talk. Qiao Wang,Zixuan Wang,Jin Zhang,Qi Zhang,Maiqing Zheng,Jie Wen,Guiping Zhao,Qinghe Li. 2022

[7]Effects of maternal Escherichia coli lipopolysaccharide exposure on offspring: insights from lncRNA analysis in laying hens. Liu, Lei,Wang, Wei,Adetula, Adeyinka Abiola,Yan, Zhixun,Liu, Huagui,Yu, Ying,Chu, Qin. 2025

[8]MicroRNAs in the immune organs of chickens and ducks indicate divergence of immunity against H5N1 avian influenza. Li, Zezhong,Zhang, Jinyu,Liu, Yinuo,Guo, Jiang,Zhang, Yonghong,Xing, Shenyang,Sun, Boxing,Zhao, Zhihui,Li, Zezhong,Zhang, Jinyu,Liu, Yinuo,Guo, Jiang,Zhang, Yonghong,Xing, Shenyang,Sun, Boxing,Zhao, Zhihui,Lu, Chunyan,Su, Jiazi,Guan, Yuntao,Li, Yanbing.

[9]MicroRNA transcriptome analysis of chicken embryo fibroblast cells infected with Newcastle disease virus variants. Weiwei Liu,Zejun Xu,Siyuan Wang,Cuiping Song,Xusheng Qiu,Lei Tan,Yingjie Sun,Ying Liao,Guijun Wang,Xiufan Liu,Chan Ding. 2023

[10]噬菌体展示技术分析鸡贫血病毒VP1蛋白抗原结构特征. 王晓艳,高玉龙,高宏雷,付朝阳,邓小芸,林欢,祁小乐,王笑梅. 2007

[11]GlobalgeneexpressionprofileofchickenspleentissueatalaterstageofIBVinfectionrevealspathogenesisandimmunemechanisms. FengCong,ZongxiHan,FengjieWang,YuhaoShao,XiangangKong,ShengwangLiu. 2014

[12]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[13]Cytotoxicity of mequindox and its metabolites in HepG2 cells in vitro and murine hepatocytes in vivo. Liu, Yingchun,Jiang, Wei,Chen, Yongjun,Zeng, Peng,Xue, Feiqun,Wang, Quan,Liu, Yanyan.

[14]Enantioselective metabolism and toxic effects of metalaxyl on primary hepatocytes from rat. Wang, Xinru,Zhu, Wentao,Zhang, Ping,Wang, Yao,Zhou, Zhiqiang,Qiu, Jing.

[15]Mir-15/16 Mediate Crosstalk Between The M.apk And Wnt/Beta-Catenin Pathways D uring Hepatocyte Differentiation From Amniotic Epithelial Cells. Bai, CY, Zhang, HW, Zhang, XY, Yang, WC, Li, XC, Gao, YH. 2019

[16]Soy-whey dual-protein alleviates osteoporosis of ovariectomized rats via regulating bone fat metabolism through gut-liver-bone axis. Zhang J.,Zhang Q.,Liu H.,Liu X.,Yu Y.,Han D.,He X.,Zeng P.,Wang J.. 2022

[17]Transcriptome Comparison Reveals the Difference in Liver Fat Metabolism between Different Sheep Breeds. Taotao Li,Meilin Jin,Xiaojuan Fei,Zehu Yuan,Yuqin Wang,Kai Quan,Tingpu Wang,Junxiang Yang,Maochang He,Caihong Wei. 2022

[18]Editorial: Fat Metabolism and Deposition in Poultry: Physiology, Genetics, Nutrition and Interdisciplinary Research, Volume I. Sami Dridi,Craig W. Maynard,Jie Wen,Elizabeth R. Gilbert. 2022

[19]MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver. Xiaojuan Fei,Meilin Jin,Zehu Yuan,Taotao Li,Zengkui Lu,Huihua Wang,Jian Lu,Kai Quan,Junxiang Yang,Maochang He,Tingpu Wang,Yuqin Wang,Caihong Wei. 2023

[20]动物繁殖相关lncRNA的研究新进展. 贺小云,狄冉,胡文萍,王翔宇,刘秋月,储明星. 2016

作者其他论文 更多>>