数字农科院2.0

Single-cell RNA sequencing reveals a cellular atlas of the sheep testis

文献类型： 外文期刊

作者： Wu, Yi;Zhu, Lina;An, Xuejiao;Yue, Yaojing

作者机构：

关键词： Sheep;Testis;Spermatogenesis;ScRNA-seq

期刊名称： GENOMICS

ISSN： 0888-7543

年卷期： 2025 年 118 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Spermatogenesis is a highly regulated biological process involving various cell types, making the accurate identification and classification of different cell populations challenging. Single-cell RNA sequencing (scRNAseq) has emerged as a powerful tool for resolving cellular heterogeneity at high resolution. In this study, 10x Genomics ChromiumTM scRNA-seq was employed to analyze 15,826 testicular cells from three 6-month-old sexually mature Hu sheep, and a high-resolution single-cell transcriptomic atlas was generated. Specifically, six somatic cell subtypes and five germ cell subtypes were identified, which revealed the differentiation trajectory of spermatogenesis. Notably, pseudo-time analysis suggested that Leydig cells and peritubular myoid cells may originate from a common progenitor lineage in sheep. Highly expressed genes were enriched in pathways such as cAMP signaling, PI3K-Akt, and ECM-receptor interaction. Furthermore, immunohistochemical analysis validated specific marker proteins for certain cell types. Collectively, these findings provide novel insights into spermatogenesis and serve as a valuable reference for the in vitro culture of male germ cells.

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