Large-fragment insertion activates gene GaFZ (Ga08G0121) and is associated with the fuzz and trichome reduction in cotton (Gossypium arboreum)
文献类型: 外文期刊
作者: Xiaoyang Wang;Yuchen Miao;Yingfan Cai;Gaofei Sun;Yinhua Jia;Song Song;Zhaoe Pan;Yuanming Zhang;Liyuan Wang;Guoyong Fu;Qiong Gao;Gaoxiang Ji;Pengpeng Wang;Baojun Chen;Zhen Peng;Xiaomeng Zhang;Xiao Wang;Yi Ding;Daowu Hu;Xiaoli Geng;Liru Wang;Baoyin Pang;Wenfang Gong;Shoupu He;Xiongming Du
作者机构:
关键词: fuzzless mutant;genome-wide association study;Gossypium arboreum;structural variation
期刊名称: Plant Biotechnology Journal
ISSN: 1467-7644
年卷期: 2021 年 19.0 卷 6.0 期
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收录情况: JCR(2021版)
摘要: Cotton seeds are typically covered by lint and fuzz fibres. Natural ‘fuzzless’ mutants are an ideal model system for identifying genes that regulate cell initiation and elongation. Here, using a genome-wide association study (GWAS), we identified a ~ 6.2 kb insertion, larINDELFZ, located at the end of chromosome 8, composed of a ~ 5.0 kb repetitive sequence and a ~ 1.2 kb fragment translocated from chromosome 12 in fuzzless Gossypium arboreum. The presence of larINDELFZ was associated with a fuzzless seed and reduced trichome phenotypes in G. arboreum. This distant insertion was predicted to be an enhancer, located ~ 18 kb upstream of the dominant-repressor GaFZ (Ga08G0121). Ectopic overexpression of GaFZ in Arabidopsis thaliana and G. hirsutum suggested that GaFZ negatively modulates fuzz and trichome development. Co-expression and interaction analyses demonstrated that GaFZ might impact fuzz fibre/trichome development by repressing the expression of genes in the very-long-chain fatty acid elongation pathway. Thus, we identified a novel regulator of fibre/trichome development while providing insights into the importance of noncoding sequences in cotton. © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
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