数字农科院2.0

Identification, distribution pattern of IS231 elements in Bacillus thuringiensis and their phylogenetic analysis

文献类型: 外文期刊

作者: Liu, JJ;Song, FP;Shu, CL;Qiu, JZ;Guan, X;Huang, DF;Zhang, J

作者机构:

关键词: Bacillus thuringiensis;insertion sequences;IS231;phylogenetic analysis;evolution;INSERTION-SEQUENCE;NUCLEOTIDE-SEQUENCE;CEREUS;ORGANIZATION;SPECIFICITY;ANTHRACIS;CLONING;FAMILY;STRAIN

期刊名称: FEMS MICROBIOLOGY LETTERS

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In order to better understand the fundamental biology of Bacillus thuringiensis, a single oligonucleotide primer (5'-CATSSCCATCAASYTAAVR-3') was used to investigate the distribution pattern of IS231 elements in B. thuringiensis by PCR. The results indicated that IS231 elements appeared in 20 standard strains and 107 of 111 China isolates. Three novel IS231, IS231J, IS231O and 1S231Q, five variants and a mobile insertion cassette MICBth4 were cloned from eight standard strains of B. thuringiensis, respectively. Interestingly, BLAST analysis revealed that the 5' end of novel IS231J shared 99% identity in 495-bp with a DNA segment adjacent to the 3' end of B. thuringiensis vip1Ac gene (GenBank Accession No. AY245547). Two phylogenetic trees of IS231 elements were constructed and analyzed by neighbor-joining and UPGMA methods from PHYLIP 3.6b program, respectively. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

分类号: Q93

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