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Nanoparticle vaccines based on the receptor binding domain of porcine deltacoronavirus elicit robust protective immune responses in mice

文献类型： 外文期刊

作者： Yuanhong Wang;Junhan Song;Xiaoying Deng;Junna Wang;Miao Zhang;Yun Liu;Pan Tang;Huili Liu;Yanjun Zhou;Guangzhi Tong;Guoxin Li;Lingxue Yu

作者机构：

关键词： ferritin;nanoparticle vaccine;porcine deltacoronavirus;RBD;SpyTag/SpyCatcher

期刊名称： Frontiers in Immunology

ISSN： 1664-3224

年卷期： 2024 年 15 卷

页码：

收录情况： SCIE（2024版）

摘要： Background: Porcine deltacoronavirus (PDCoV), a novel swine enteropathogenic coronavirus, challenges the global swine industry. Currently, there are no approaches preventing swine from PDCoV infection. Methods: A new PDCoV strain named JS2211 was isolated. Next, the dimer receptor binding domain of PDCoV spike protein (RBD-dimer) was expressed using the prokaryotic expression system, and a novel nanoparticle containing RBD-dimer and ferritin (SC-Fe) was constructed using the SpyTag/SpyCatcher system. Finally, the immunoprotection of RBD-Fe nanoparticles was evaluated in mice. Results: The novel PDCoV strain was located in the clade of the late Chinese isolate strains and close to the United States strains. The RBD-Fe nanoparticles were successfully established. Immune responses of the homologous prime-boost regime showed that RBD-Fe nanoparticles efficiently elicited specific humoral and cellular immune responses in mice. Notably, high level PDCoV RBD-specific IgG and neutralizing antibody (NA) could be detected, and the histopathological results showed that PDCoV infection was dramatically reduced in mice immunized with RBD-Fe nanoparticles. Conclusion: This study effectively developed a candidate nanoparticle with receptor binding domain of PDCoV spike protein that offers protection against PDCoV infection in mice.

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