数字农科院2.0

HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon

文献类型： 外文期刊

作者： Qin, Tong;Liang, Xiuan;Zhong, Chen;Zhang, Zhiheng;Wang, Jingjue;Shi, Jiahuan;Huang, Jingjing;Chen, Dong;Zhao, Wei;Wang, Mengyang;Zhao, Jianlong;He, Yongqiang;Yang, Shanshan;Chai, Ali;Zhang, Xiaoxiao

作者机构：

关键词： ClRAR1;harpin;HrpW;Paracidovorax citrulli;plant immunity

期刊名称： MOLECULAR PLANT PATHOLOGY

ISSN： 1464-6722

年卷期： 2025 年 26 卷 6 期

页码：

收录情况： SCIE（2025版）

摘要： Bacterial fruit blotch (BFB), caused by Paracidovorax citrulli, severely threatens watermelon production. This study investigates the role of HrpW, an atypical harpin in P. citrulli AAC00-1, in bacterial virulence and host immune modulation. Bioinformatics analysis revealed HrpW harbours a unique signal peptide and structural features distinct from other harpins. Deletion of hrpW impaired bacterial motility, biofilm formation and virulence, while complementation restored these traits. HrpW suppressed reactive oxygen species (ROS) bursts and mitogen-activated protein kinase (MAPK) activation in plants but failed to induce programmed cell death (PCD). Crucially, HrpW inhibited the hypersensitive response (HR) triggered by P. citrulli in non-host tobacco, with Delta hrpW mutant inducing premature HR. RNA-seq analysis demonstrated HrpW downregulated ClRAR1 expression. Silencing ClRAR1 or ClSGT1 compromised watermelon resistance, and notably, ClRAR1-silenced plants exhibited PCD upon HrpW treatment, indicating HrpW-mediated PCD suppression is RAR1-dependent. Importantly, HrpW triggered ubiquitin-dependent degradation of ClRAR1 and independently disrupted ClRAR1-ClSGT1 binding, thereby suppressing effector-triggered immunity (ETI). HrpW translocated into plant cells via the type III secretion system (T3SS), as confirmed by CyaA assays. Intriguingly, low concentrations of HrpW enhanced watermelon resistance to BFB, while high concentrations promoted disease progression, revealing a concentration-dependent duality. This study unveils HrpW as a multifunctional virulence factor that modulates bacterial fitness, suppresses HR and manipulates host immunity via RAR1 targeting. These findings expand our understanding of harpin-mediated pathogenicity and offer insights for sustainable BFB management strategies.

分类号：

  • 相关文献

[1]葡聚烯糖和Harpin蛋白对两种番茄病害的防治效果. 张拥华,李世东,刘杏忠. 2003

[2]表达Harpin蛋白的转基因马铃薯降低晚疫病斑生长率. 李汝刚,范云六. 1999

[3]Reduction of lesion growth rate of late blight plant disease in transgenic potato expressing harpin protein. Li, RG,Fan, YL.

[4]A novel probe for harpin receptor in nonhost plants: Monoclonal anti-idiotypic antibodies as internal images of Harpin(Xoo) active sites. Chen, Defeng,Hu, Baishi,Qian, Guoliang,Gu, Chunyan,Fan, Jiaqin,Liu, Fengquan,Zhang, Qi. 2008

[5]Identification of phenolic compounds that suppress the virulence of Xanthomonas oryzae on rice via the type III secretion system. Tian, Fang,Li, Jianyu,Chen, Huamin,Yang, Fenghuan,He, Chenyang,Hutchins, William,Yuan, Xiaochen,Yang, Ching-Hong,Cui, Zining.

[6]Chromatin versus pathogens: the function of epigenetics in plant immunity. Wang, Guo-Liang,Wang, Guo-Liang. 2015

[7]Plant innate immunity in rice: a defense against pathogen infection. Wang, Guo-Liang,Wang, Guo-Liang. 2016

[8]A pair of light signaling factors FHY3 and FAR1 regulates plant immunity by modulating chlorophyll biosynthesis. Wang, Wanqing,Tang, Weijiang,Ma, Tingting,Lin, Rongcheng,Niu, De,Jin, Jing Bo,Wang, Haiyang,Lin, Rongcheng. 2016

[9]Insights into the Biocontrol Function of a Burkholderia gladioli Strain against Botrytis cinerea. Wang, Dan,Luo, Wan-Zhen,Zhang, Dan-Dan,Li, Ran,Kong, Zhi-Qiang,Song, Jian,Dai, Xiao-Feng,Alkan, Noam,Chen, Jie-Yin. 2023

[10]Identification And Characterization Of Plasmodiophora B.rassicae Primary Infection Effector C andidates That Suppress Or Induce Cell Death In Host And Nonhost Plants. Chen, W, Li, Y, Yan, RB, Xu, L, Ren, L, Liu, F, Zeng, LY, Yang, H, Chi, P, Wang, XZ, Chen, KR, Ma, DF, Fang, XP. 2019

[11]DNA-Binding Activity of CAMTA3 Is Essential for Its Function: Identification of Critical Amino Acids for Its Transcriptional Activity. Kasavajhala V.S.K. Prasad,Amira A.E. Abdel-Hameed,Qiyan Jiang,Anireddy S.N. Reddy. 2023

[12]Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton. Li, Chi,Qin, Jun,Huang, Yingqi,Shang, Wenjing,Chen, Jieyin,Klosterman, Steven J.,Subbarao, Krishna V.,Hu, Xiaoping. 2023

[13]New insights into defense responses against Verticillium dahliae infection revealed by a quantitative proteomic analysis in Arabidopsis thaliana. Wu, Min,Li, Qiulin,Xia, Guixian,Zhang, Yongshan,Wang, Fuxin. 2022

[14]Nuclear exportin 1 facilitates turnip mosaic virus infection by exporting the sumoylated viral replicase and by repressing plant immunity. Mingzhen Zhang,Pan Gong,Linhao Ge,Zhaoyang Chang,Xiaofei Cheng,Xueping Zhou,Aiming Wang,Fangfang Li. 2021

[15]Defects in plant immunity modulate the rates and patterns of RNA virus evolution. Navarro, Rebeca,Ambros, Silvia,Butkovic, Anamarija,Carrasco, Jose L.,Gonzalez, Ruben,Martinez, Fernando,Wu, Beilei,Elena, Santiago F.. 2022

[16]Lignin and Its Pathway-Associated Phytoalexins Modulate Plant Defense against Fungi. Vincent Ninkuu,Jianpei Yan,Zenchao Fu,Tengfeng Yang,James Ziemah,Matthias S. Ullrich,Nikolai Kuhnert,Hongmei Zeng. 2023

[17]The Conserved Cysteine-Rich Secretory Protein MaCFEM85 Interacts with MsWAK16 to Activate Plant Defenses. Ni Cai,Xiangqun Nong,Rong Liu,Mark Richard McNeill,Guangjun Wang,Zehua Zhang,Xiongbing Tu. 2023

[18]A VQ-motif-containing protein fine-tunes rice immunity and growth by a hierarchical regulatory mechanism. Hao Z.,Tian J.,Fang H.,Fang L.,Xu X.,He F.,Li S.,Xie W.,Du Q.,You X.,Wang D.,Chen Q.,Wang R.,Zuo S.,Yuan M.,Wang G.-L.,Xia L.,Ning Y.. 2022

[19]Unlocking antagonistic potential of Bacillus amyloliquefaciens KRS005 to control gray mold. Hong Yue Qi,Dan Wang,Dongfei Han,Jian Song,Muhammad Ali,Xiao Feng Dai,Xiao Jun Zhang,Jie Yin Chen. 2023

[20]Two Magnaporthe appressoria-specific (MAS) proteins, MoMas3 and MoMas5, are required for suppressing host innate immunity and promoting biotrophic growth in rice cells. Gong, Ziwen,Ning, Na,Li, Zhiqiang,Xie, Xin,Wilson, Richard A.,Liu, Wende. 2022

作者其他论文 更多>>