数字农科院2.0

CRISPR/Cas9-mediated knockup of OsDREB1C enhances rice yield without compromising grain quality

文献类型： 外文期刊

作者： Luo Y#; Zhan X.; Zhang Y; Wang B; Wang G; Zhang Y; Li G; Liu Q; Shen X.; Chen D; Hong Y; Wu W; Ye G; Cheng S; Pan G*; Cao L*

关键词： CRISPR-Cas9;;desirable phenotypes;;rice

期刊名称： Plant Communications

ISSN： 2590-3462

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度）

摘要： CRISPR-Cas9 genome editing has revolutionized plant breeding by enabling precise genetic modifications in staple crops such as rice and wheat . Through targeted gene knockout or knockdown, this echnology has demonstrated remarkable success in improving yield potential, stress resistance, and nutritional quality . In rice breeding, CRISPR-Cas9 has been successfully used to induce genomic structural variation (SV) and enhance pesticide resistance). However, inversions, an often-overlooked type of SV, remain rarely reported despite their potential for crop improvement Inversions occur when CRISPR-Cas9 induces doublestrand breaks at the promoter and coding regions of two oppositely oriented genes on the same chromosome. This inversionstyle rearrangement can swap the genes’ promoters, altering their expression, and potentially enhancing desirable phenotypes such as yield.

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