数字农科院2.0

Alkaline mineral complex water protects piglets against viral infection by driving cholesterol consumption through the GPAT2/SULT2A1 axis

文献类型： 外文期刊

作者： 姚鑫;;李诺娃;;刘莹莹;;Kanwar Kumar Malhi;;张甜甜;;赵阳;;李慧昕;;李金龙

关键词： Alkaline mineral complex; Viruses; GPAT2/SULT2A1 axis; Lipid metabolism; Antiviral

期刊名称： Science China-Life Sciences

ISSN： 1674-7305

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Our previous work revealed that the anti-diarrhea effects of alkaline mineral complex (AMC) water improve metabolism and protect the gut during weaning stress. However, whether AMC water can inhibit viral replication and treat viral diarrhea is unknown. The aim of this study was to explore the ability of AMC water to improve nutrient metabolism and protect against infection. In this study, porcine epidemic diarrhea virus (PEDV) or porcine deltacoronavirus (PDCoV) were used as RNA model viruses, and pseudorabies virus (PRV) or porcine circovirus (PCV) were used as DNA model viruses. Compared with those in the infected group, the virus content in the piglets fed AMC water was reduced, and the intestinal mucosal barrier was repaired. Transcriptome and metabolome results revealed that AMC water regulated lipid metabolism through GPAT2, DGKA, OAT3, FXR, LIPC and SULT2A1. Further studies showed that glycerol, cholesterol, and bilirubin levels increased after viral 42 infection, and that AMC water inhibited cholesterol content and promoted bile acid synthesis. In a cellular model, AMC water reduced lipid droplet density by activating the glycerolipid and bile secretion pathways of the GPAT2/SULT2A1 axis. In addition,5 knockdown of DGKA and overexpression of SULT2A1 significantly affected the expression of the GPAT2/SULT2A1 axis, and the expression of viral proteins colocalized with lipid droplets was significantly decreased. Our findings suggest that AMC water promotes cholesterol metabolism by activating the GPAT2/SULT2A1 axis, inhibiting viral infection in piglets. This study provides theoretical support for the use of nutritional regulation to inhibit viral infection and provides a new method for antiviral therapy.

分类号：

  • 相关文献

[1]Site-selective proteolytic cleavage of plant viruses by photoactive chiral nanoparticles. Gao R., Xu L., Sun M., Xu M., Hao C., Guo X., Colombari F.M., Zheng X., Král P., de Moura A.F., Xu C., Yang J., Kotov N.A., Kuang H.. 2022

[2]Genome-Wide Analysis Of Differentially Expressed G.enes And The Modulation O f Pedv Infection In Vero E6 Cells. Zhang, HW, Liu, QF, Su, WW, Wang, JK, Sun, YR, Zhang, JF, Shang, K, Chen, ZH, Cheng, SP, Wu, H. 2018

[3]The long non-coding RNA LNC_000397 negatively regulates PRRSV replication through induction of interferon-stimulated genes. Zhang, Jing,Gan, Lipeng,Sun, Pu,Wang, Jian,Li, Dong,Cao, Yimei,Fu, Yuanfang,Li, Pinghua,Bai, Xingwen,Li, Kun,Ma, Xueqing,Bao, Huifang,Chen, Yingli,Zhang, Jie,Liu, Zaixin,Lu, Zengjun. 2022

[4]In Vitro And In Vivo A.ntiviral Activity Of Mizoribine A gainst Foot-And-Mouth Disease Virus. Li, SF, Gong, MJ, Sun, YF, Shao, JJ, Zhang, YG, Chang, HY. 2019

[5]Type I Interferons: Distinct Biological A.ctivities And Current Applications F or Viral Infection. Li, SF, Gong, MJ, Zhao, FR, Shao, JJ, Xie, YL, Zhang, YG, Chang, HY. 2018

[6]Isobavachalcone inhibits Pseudorabies virus by impairing virus-induced cell-to-cell fusion. Liu, Tian-Xin,Wang, Tong-Yun,Tang, Yan-Dong,Wang, Yu,Wei, Ping,Wang, Yu. 2020

[7]Effects Of Clostridium Butyricum On G.rowth Performance, Lipid Metabolism A nd The Caecal Microecological Environment Of Broilers. Shi, S. R.,Ding, Xiao,Yang, Z. B.,Zhao, Xu,Shao, Dan,Shen, Yiru,Shi, S. R.. 2017

[8]Efforts Of Clostridium Butyricum On B.reast Muscle Lipid Metabolism O f Broilers. Zhao, Xu,Zhang, Shan,Yang, Zaibin,Shi, Shourong,Shen, Yiru,Ding, Xiao. 2018

[9]Toxic Effects Of Boscalid In A.dult Zebrafish (Danio Rerio) O n Carbohydrate And Lipid Metabolism. Qian, L, Zhang, J, Chen, XG, Qi, SZ, Wu, PZ, Wang, C, Wang, CJ. 2019

[10]Chitooligosaccharide Promotes Immune Organ Development I.n Broiler Chickens And R educes Serum Lipid Levels. Li, SH, Jin, EH, Qiao, EM, Wu, GZ, Li, K. 2017

[11]Pnpla5-Knockout Rats Induced By Crispr/Cas9 E.xhibit Abnormal Bleeding And L ipid Level. Liu, Y, Gao, Q, Zhang, X, Huang, L, Xu, K, Hu, YQ, Liu, L, Mu, YL, Li, K. 2017

[12]Lipidomics provides novel insights into understanding the bee pollen lipids transepithelial transport and metabolism in human intestinal cells. 李强强,,薛晓锋,,王凯,,吴黎明. 2020

[13]Transcriptome Analysis Reveals Mechanism Underlying The Differential Intestinal Functionality Of Laying Hens In The Late Phase And Peak Phase Of Production. Wang, WW, Wang, J, Zhang, HJ, Wu, SG, Qi, GH. 2019

[14]Mechanisms Of Developmental Toxicity In Z.ebrafish Embryos (Danio Rerio) I nduced By Boscalid. Qian, L, Cui, F, Yang, Y, Liu, Y, Qi, SZ, Wang, CJ. 2018

[15]Developmental Toxicity By Thifluzamide In Z.ebrafish (Danio Rerio): Involvement O f Leptin. Wang, Donghui,Yang, Yang,Zheng, Yongquan,Dong, Fengshou,Xu, Jun,Liu, Xingang,Wu, Xiaohu. 2019

[16]Postharvest Metabolomic Changes In Pyrus U.ssuriensis Maxim. Wild Accession ' Zaoshu Shanli'. Xu, JY, Zhang, Y, Qi, D, Huo, HL, Dong, XG, Tian, LM, Zhang, XS, Liu, C, Cao, YF. 2018

[17]Conjugated Linoleic Acid Regulates Lipid M.etabolism Through The Expression O f Selected Hepatic Genes In Laying Hens. Wang, SH, Wang, WW, Zhang, HJ, Wang, J, Chen, Y, Wu, SG, Qi, GH. 2019

[18]Atmospheric Ammonia Alters Lipid Metabolism-Related G.enes In The Livers O f Broilers (Gallus Gallus). Sa, RN, Xing, H, Luan, SJ, Sun, YB, Sun, CY, Zhang, HF. 2018

[19]Identification And Characterization Of Circrnas O.f Two Pig Breeds A s A New Biomarker In Metabolism-Related Diseases. Li, A, Huang, WL, Zhang, XX, Xie, LL, Miao, XY. 2018

[20]Effects Of Dietary Alpha-Linolenic Acids O.n Growth Performance, Lipid M etabolism And Antioxidant Responses Of Juvenile Russian Sturgeon Acipenser Gueldenstaedtii. Wang, XD, Li, Q, Wang, JH, Li, EC, Qin, JG, Chen, LQ. 2019

作者其他论文 更多>>