Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus
文献类型： 外文期刊
作者： Wang J.;Sun C.;Hu Z.;Wang F.;Chang J.;Gao M.;Ye D.;Jia Q.;Zou H.;Willems L.;Jiang Z.;Yin X.
作者机构：
关键词： Bovine leukemia virus;Competitive ELISA;Monoclonal antibody;p24 protein;Serological detection
期刊名称： International Journal of Biological Macromolecules
ISSN： 0141-8130
年卷期： 2024 年 267 卷
页码：
收录情况： SCIE（2024版） ; ; EI（2024版）
摘要： Infection with bovine leukemia virus (BLV) leads to enzootic bovine leukosis, the most prevalent neoplastic disease in cattle. Due to the lack of commercially available vaccines, reliable eradication of the disease can be achieved through the testing and elimination of BLV antibody-positive animals. In this study, we developed a novel competitive ELISA (cELISA) to detect antibodies against BLV capsid protein p24. Recombinant p24 protein expressed by Escherichia coli, in combination with the monoclonal antibody 2G11 exhibiting exceptional performance, was used for the establishment of the cELISA. Receiver-operating characteristic curve analysis showed that the sensitivity and specificity of the assay were 98.85 % and 98.13 %, respectively. Furthermore, the established cELISA was specific for detecting BLV-specific antibodies, without cross-reactivity to antisera for six other bovine viruses. Significantly, experimental infection of cattle and sheep with BLV revealed that the cELISA accurately monitors seroconversion. In a performance evaluation, the established cELISA displayed a high agreement with Western blotting and the commercial BLV gp51 cELISA kit in the detection of 242 clinical samples, respectively. In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect. © 2024 The Author(s)
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